The Expression And Its Meaning Of MIF And VEGF-C In Human Normal Mammary Gland Cells And Breast Canner MDA-MB-231、MCF-7Cell

Objective: Research the expression of VEGF-C and MIF in humannormal breast cells (HCC1937)、breast cancer MDA-MB-231and MCF-7cell, Analysis of the MIF expression in breast cancer cells and normalcells,Analysis of the VEGF-C expression in breast cancer cells and normalcells,And analysis of the MIF and the VEGF-C expressed in breast cancertissues and normal breast tissue.Methods: In vitro human normal breast cells (HCC1937) and breastcancer MDA-MB-231, the MCF-7cell.From the training set of total proteinin the cell, using Western blot test normal breast cells (HCC1937) and MDA-MB-231breast cancer, MIF in MCF-7cell and the expression of VEGF-Cprotein;From the total training group of cells of mRNA, and using rt-pcr testnormal breast cells (HCC1937) and MDA-MB-231breast cancer, MCF-7cell of MIF and VEGF-C mRNA expression level.Finally, MIF groups of cellsand VEGF-C protein and mRNA expression level for statistical analysis.Results:Detection the expression of MIF and VEGF-C protein By usingthe method of Western blot, Beta actin was used as internal calibrationresults,Found there are lower expression of MIF and VEGF-C protein innormal breast cells (HCC1937) protein, but there are high expression of MIFand VEGF-C protein in MDA-MB-231breast cancer and in MCF-7cell,The results are shown in figure1.The Image analysis software Pro4.5measuring was use to value beta actin MIF and VEGF-C protein in normalbreast cells (HCC1937) and MDA-MB-231breast cancer, MCF-7cell ofWestern blot grey value in the band,The experimental results to take three times the average of the experimentaldata,Statistical analysis results was said with,The Express of MIF andVEGF-C protein in normal breast cells (HCC1937) and MDA-MB-231breast cancer is statistically significant difference（sP<0.05,r=0.902）, by the testsof the expression of MIF and VEGF-C protein molecular in normal breast cells(HCC1937) and MDA-MB-231breast cancer, MCF-7cell, We found that theexpression of MIF and VEGF-C protein are high in breast cancer MDA-MB- 231, MCF-7cell than in normal breast cells (HCC1937), to validate ourconclusion At the genetic level,We did rt-pcr to further testing the MIF mRNAand VEGF-C mRNA in normal breast cells (HCC1937), breast cancer MDA-MB-231and in MCF-7cell,Beta actin was used as internal calibrationresults,By Joan1.0%fat sugar electrophoresis,Found there are lower expressionof MIF and VEGF-C mRNA in normal breast cells (HCC1937) mRNA, butthere are high expression of MIF and VEGF-C mRNA in MDA-MB-231breast cancer and in MCF-7cell,MIF mRNA in normal breast cells (HCC1937)-c and VEGF mRNA low table,The Image analysis software Pro4.5measuringwas use to value beta actin MIF and VEGF-C mRNA in normal breast cells(HCC1937) and MDA-MB-231breast cancer, MCF-7cell of Western blotgrey value in the band,The experimental results to take three times the averageof the experimental data,Statistical analysis results was said with,TheExpress of MIF and VEGF-C mRNA in normal breast cells (HCC1937) andMDA-MB-231、MCF-7breast cancer is statistically significant differences（P<0.05,r=0.817）Conclusions: MIF and VEGF-C in breast cancer tissue increased andpositively correlated, but in normal breast cells(HCC1937) assumes the lowerexpression of MIF and VEGF-C.Between MIF and VEGF-C there may besome regulatory mechanism, promote the over expression in breast cancer,further study of regulatory mechanism for the treatment of patients with breastcancer and the prevention of cancer metastasis plays an important role.