| Objective:Use the lever principle to create the T13Spinal cord syndrome on the rat on the basis of the typical McBride model.Evaluate the influence of the prescription "Fructus aurantii liquorice decoction" created by Mr. Gong,who combines his dozens of years of clinical prescription experience with the compatibility principle of "Large Chengqi Decoction",with the title of the nationally famous TCM doctor,and one of national apprentice tutors,on the expression of SOD and MDA in the spinal cord of the damage section,the volume of6-Keto-PGFla and TXB2in the serum,and explore the therapy and mechanism of "Fructus aurantii liquorice decoction" on the model.Methods:1.Take108male SD rats which have adjusted to the environment for3days,and96to create the T13Spinal cord syndrome on the rat based on the typical McBride model.Randomly divide them into8groups(n=12),which is the model group,the positive medicine (methylprednisolone) group,and Chinese medicine group(1、2、3、4、5、6)group,and the other12as the fake operation group,which are operated the same as the molding method,and be careful not to bite the vertebral pedicle and processus transversus with the needle holder.Administrate them instantly after the division with Fructus aurantii liquorice decoction to fill the stomach in each TCM group at the1.0g/ml-10ml/Kg rate,namely the dose of10g/kg.9mg/ml intraperitoneal injection of methylprednisolone in10ml/kg volume,which is the dose of90mg/ml,in positive medicine group,and the same dose of fresh distilled water in the model and fake operation group.The process continues14days with one time each day.After the creation,use the BBB and CBS score system respectively to perform the movement and nerve evaluation at the timelines of3h,1st,3rd,7th and14thd.2.Take45of60male SD rats in the same condition as before.The animals all wake up60minutes after the creation,and randomly divide them into3groups(n=15),which is the model,positive medicine and optimize TCM group,and the rest15as the fake operation team in the same way as before.The manipulation process is the same as before,only continuing28days at one time each day.Evaluate the movement function with BBB score system at the timeliness of the3th,14th and28th day after operation.Take5rats each group at the timeliness of the3th,14th and28th day after operation,and deprive them of the same segment of the spinal cord tissue,half with the HE dying to execute pathology observation,and half with colorimetric method to detect the contents of SOD and oxygen free radical degradation mainly product MDA.At the same point,take the serum of rats and the method of ELISA to detect the6-Keto-PGFla and TXB2concentration.Result:1.The rats in the model groups all gradually rise with the extension of time.Of all,the4groups of positive medicine and TCM share larger rising amplitude at the end of each observation point compared with the other ones. After building3,7and14days,the BBB rating of the4positive medicine treatment and TCM formula groups increased significantly compared with model group,which proved statistically significance(p<0.05).7and14days after building,the score of traditional Chinese medicine formula2and3was also significantly higher than that of model group,which proved statistically significant(p<0.05).But the BBB score of the2groups are both lower than the4TCM treatment groups,implying the4th TCM group treats better than the rest ones.The CBS scores of the animals of before spinal cord syndrome model groups(i.e. the treatment groups)all reduced gradually over time, of all, positive medicine group and the4th traditional Chinese medicine treatment group share larger range at each observation point than other treatment groups.7and14days after building,the CBS ratings of the positive-controlled(methyl prednisolone)treatment group and the4th Chinese traditional medicine increase significantly compared with model control group,which prove statistically significance(p<0.05).14days after building,the2nd traditional Chinese medicine also scores significantly higher than that of model control group,which prove statistically significance(p<0.05).But the CBS average score in treatment groups is lower than that of the4th traditional Chinese medicine group,implying that the4th traditional Chinese medicine group treats better.2.After building for3,14and28days,the BBB rating of positive medi cine treatment group and Chinese traditional medicine are higher compared with model control group obviously,which prove statistically significant differ ence(p<0.05),implying the protective effect of the4th Chinese medicine group movement function injury caused by spinal cord injury repeatedly sustains stable for28days,further illustrating that it can promote the repair of spinal cord injury.Using the optimized fructus aurantii liquorice decoction prescript ion after building3,14and28days to observe shape variation of spinal cor d,changes of gray matter of nerve cell number,nerve cell morphological,syrin gomyelia,and glial scar formation etc.Judging from various aspects, the previ ous all have different degrees of mitigation and improvement.Compare model group with fake operation group,after buliding3,14and28days,the SOD c omponent of the injured spinal cord reduces,with the MDA rises,and exists s ignificant difference(P<0.05).Compare positive medicine group with model on e,after building3and14days,the SOD in the injured spinal cord rises,and exists significant difference(P<0.05).As to28days,the SOD falls and rises a nd exists not so much significant difference(P>0.05).After buliding3,14and28days,the MDA in the injured spinal cord reduces,and exists no significant difference(P>0.05).Compare TCM with model group:after building3days,th e SOD fall-rises,existing no significant difference(P>0.05).14and28days,the SOD fall-rises,and exists significant difference(P<0.05).After3,14and28da ys,the MDA reduces,and exists no significant difference(P>0.05).It implies th at SOD in the injured spinal cord gradually rises with the delay of the med icine’s intervention in the optimized fructus aurantii liquorice decoction presc ription group,but the MDA gradually reduces.After14and28days,in the op timized fructus aurantii liquorice decoction prescription group,the intervention time point executes more significantly than the model group as to the rise of SOD.After building3,14and28days,compare with fake operation group,6-Keto-PGF1α of model group significantly reduces.and TXB2rises,which pr ove statistical significance (P<0.05).Compare positive medicine with model g roup,after building3,14and28days,6-Keto-PGF1α rises,which is more obvi ous after14days,proving statistical significance(P<0.05).On the contrary,TXB2reduces,which is more obvious after14days,proving statistical significance (P<0.05).Compare TCM with model group,after building3,14and28days,6-Keto-PGF1α rises,which is more obvious after14days,proving statistical si gnificance(P<0.05).On the contrary,TXB2reduces,which is more obvious after14and28days,proving statistical significance (P<0.05).It implies that the ti me point of the intervention of6-Keto-PGF1α in the rats belonging to the optimized fructus aurantii liquorice decoction prescription group all rises,of a ll,the14th day intervention time for the increase of6-Keto-PGF1α content w orks better than the model group at the same time.And in the plasma of th e large rat,TXB2gradually reduces with the delay of the medicine’s interfer ence time,of which,the14th and28th day intervention time for the increase o f TXB2content works better than the model group at the same time.Conclusion:1.The optimize fructus aurantii liquorice decoction prescription (4th TCM formula:fructus aurantii20g,liquorice5g,angelica25g;rhizoma zedo ariae10g,rhubarb30g,mirabilite15g)has protective effect on the movement a nd nerve function injury caused by the spinal damage,and also can promotes the repair of the spinal cord,which can be taken to study the possible mec hanism of fructus aurantii liquorice decoction as the best dose proportion on the actual efficiency.2.The optimized fructus aurantii liquorice decoction pre scription can effectively promote the pathology of the spinal cord tissues.On the one hand,it can raise the SOD in the damaged rat’s spinal cord,and red uce the MDA.On the other hand,it can reduce theTXB2in the large rat’s p1asma,and raise the6-Keto-PGF1α,which indicates it can protect and fix the vascular endothelium,and effectively recover the synthetic function of prostag landin enzyme,so as to balance the TXB2and6-Keto-PGF1α,furtherly reduc e the risk of the secondary injury after the acute spinal damage,enhance the repair of spinal tissue,and protect the injured spinal tissues.This may be on e of the mechanisms of the protective effect of the optimized fructus auranti i liquorice decoction prescription. Also the prescription has more obvious effe ct on the persistent treatment for the secondary spinal injury. |
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