Victoria Drug Gallic On Experimental IgA Nephropathytubulointerstitial Damage And NF-κB,MCP-1Expr Ession In Rats

Objective:Observe the effects of victoria drug gallic on renal tubular interstitial damage and their mechanisms in IgA nephropathy rats rat urinary protein, urinary NAG enzyme, renal pathology and renal tissue of nuclear transcription factor NF-κB (NF-κB p65), monocyte chemoattractant protein-1(MCP-1) expression by the establishment of IgA nephropathy rat model. Methods:The100healthy male SD rats were randomly divided into three groups:normal control group; IgAN group and nutgall intervention group. IgAN model was established using fed bovine serum albumin (BSA), injection of lipopolysaccharide (LPS) and carbon tetrachloride (CC14) combination method. Urine was collected after the establishment of model. Intervention groups were given high, medium and low three doses of nutgall for4weeks, normal SD rats as control. Urine red blood cells were tested by automatic biochemical analyzer, urine protein was quantitied by the method of coomassie brilliant blue, and urine NAG enzyme activity was detected using urinary protein Elisa method. NF-κB p65A and MCP1expression were detected by immunofluorescence method using kidney frozen section. Pathological changes were observed and analyzed using immunohistochemical technique and the pathological integral kidney pathological damage was quanlified. Results:(1) Urinary erythrocytes were30±4.64/ul,32.1±4.12/ul and38.8±4.61/ul in groups of gallic intervention with high, middle and low dose respectively. Urine protein was4.57±0.98g/24h,4.18±1.22g/24h and5.54±1.23g/24h in groups of gallic intervention with high, middle and low dose respectively. Urinary NAG was1.55±0.61pg/ml,1.28±0.60pg/ml and1.4±0.47pg/ml in groups of gallic intervention with high, middle and low dose respectively. Urinary red blood cells in urine were818±36.57cells/ul, protein11.14±1.67g/24h, and urinary NAG enzyme21.14±0.27pg/ml. Intervention urinary red blood cells, urine protein, urine NAG enzyme were significantly lower than the model group, the difference was statistically significant (P<0.05).(2) Expression of NF-κBp65positive area was61.39±3.51%,39.32±3.05%,42.14±1.87%and55.89±3.28%in groups of model, gallic intervention with high, middle and low dose respectively. Expression of MCP-1expression area was51.46±2.67%,29.16±2.47%,33.03±2.72%,43.05±3.66%in groups of model, gallic intervention with high, middle and low dose respectively. Intervention group was significantly reduced compared with the model group (P<0.05).Conclusions:(1) Victoria drug gallate can reduce the model rats urine red blood cell count, urine protein and urine NAG activity;(2) Victoria drug gallate can reduce IgA nephropathy rat model of tubulointerstitial pathological damage;(3) Victoria drug gallic tubulointerstitial damage mitigation action may be related with down-regulation of NF-κB, MCP-1expression.