The Experimental Study About The Treatment Of Somatostatin On SPF Mouses Infected With Intestinal Candida Albicans

Objective1. To study the effect of Somatostatin on SPF standard kunming rats’intestinal barrier function, which were infected by Candida Albicans in intestine.Method100health Kunming mouse(female or male,age about5weeks and weighing about20g)were divided into four groups randomly, and some death; at last,80divided into control group(N=20),sham model group(N=20),treatment group (N=20) and model group (N=20).The sham model group,model group and treatment group were irrigated by Streptomycin sulfate (0.4ml,100mg/ml) and cyclophosphamide(50mg/kg) Intraperitoneal injected each for five days;the control group were treated by NS at the same time.After five days,the model group and the treatment group mouse were irrigated by1.6×109Candida Albicans;the control group and the sham model group mouse were treated by NS.The treatment group mouse were injected by Somatostatin (0.2mlper,Tid)24hours later,the others were treated by NS everyday.After five days,observing the mouse(spirit and diarrhea) and killing all mouse,collecting the stool in intestine for Candida Albicans culture and ileocecal for pathology study by,testing cell apoptosis and molecular biology tests,inclonding bcl-2and Bax protein.All datas were recorded by x±S, analyzed by SPSS13.0.Results1. General observing:The control group had no change;sham model group, model group and treatment group’s rats express apathetic such as hair standing and less movement; after opening abdominal we found the color of intestine changed to pale and the creeping slowed down of sham model group rats. model group rats’ changes were much more obviously than sham model group’s,and guts flatulence, intestinal wall edema even ascites were found in model group rats. In treatmeat group by treating with Somatostatin,we found the color of intestine changes to ruddy, the creeping of intestine speed up and guts flatulence disappeared.2. Candida Albican Culturing:candida albican was found in all groups,as blue conolys in Candida Albican chromogenic medium.With CFU counting,control group and sham model group had no difference(P>0.05), more CB found in model group than sham model group(P<0.01); and less in treatment group than model group,which were treated by Somatostatin (p<0.01).3. Pathological Studing:No significantly inflammation was found in control group,but the sham model group, model group and treatment groups had different levels of inflammation in intestinal tissue, such as inflammation cell were found in intestinal tissue, intestinal villus tip off and so on, According to pathological scored about intestinal tissue we found the injury of intestinal barrier was more significantly in the sham model group than control group(P<0.05), model group was more significantly than sham model group (P<0.01);treatment group was better than model group,which were treated by Somatostatin.(P＜0.01)4. Apoptosis of intestinal cell:apoptosis of intestinal cells were found in all groups,that appeared the volume of cell changed small, paryopyknosis,chromatin agglutination and the color of nucleus were dyed to claybank; same apoptotic cell of intestine were found in control group,but sham model group more than control group(P<0.05);and the model group was more than sham model group(P<0.05);the treatment group which were treated by Somatostatin were much less than model group(P<0.01);5. Bcl-2protein expressing in intestina cells:bcl-2positive intestinal cells were found in all groups, mainly in cytoplasm; the content of Bcl-2in sham model group was more less than control group (P<0.01), model group much more less than sham model group(P<0.05),but the treatment group which treated by Somatostatin was much more than sham model group(P<0.05).6. Bax protein expressing in intestinal cells:Bax positive intestinal cells were found in all groups, mainly in cytoplasm;a less of Bax expressed in the control group, Bax expressing in sham model group were more than control group(P<0.05),but there were no difference in sham model group with model group(P>0.05) and treatment group with model group(P>0.05).Conclusions1. Somatostatin could reduce the apoptosis of intestinal cells in rats maybe with reference to decreasing Bcl-2expressing in intestinal cells.2. Somatostatin could reduce the number of candida albicans and apoptotic cell in intestine of immunocompromised rats’intestine which were treated by Streptomycin sulfate,and the injury of intestine was remission.3. Somatostatin could reduce the apoptosis of intestinal cells in rats maybe with reference to increasing Bcl-2expressing in intestinal cells.