| ObjectiveMice infected with candida albicans were treated by combining somatostatin withtraditional Chinese medicine Qingyitang, hematoxylin-eosin staining are performed tothe intestinal structure, comments were given based on pathology analysis, colonycounting value(CFU) were observed, apoptosis Bax and apoptosis gene Bcl-2weredetected by Tunel method and immunohistochemistry technology, comparing withtreatment effect of using somatostatin or Qingyitang separately, relations between thesetwo medicines and apoptosis Bax and apoptosis gene Bcl-2in the treatment of candidaalbicans infection were studied, moreover, the possibility of drug combination on thebasis of protecting intestine mucosal barrier were explored.Methods100healthy Kunming female mice in SPF level were selected, weight ranged from18g to20g，age about4weeks.20mice are taken as blank control group(N) throughrandomization，80mice were taken as model group infected by candida albicans, miceweight had no obvious difference after grouping. Two weeks later,5mice were selectedfrom control group and model group respectively, taking their intestinal structures,colony counting value was detected and HE staining method was performed. It wasconfirmed that candida albicans model had been succeeded by inspecting under opticalmicroscope. The rest of64mice in model group (11mice died during modeling) weredivided into model group and experimental group (somatostatin group, Qingyitang groupand drug combination group) at random and mice in different groups were treated bymedicines respectively.Mice in somatostatin group were injected somatostation with theconcentration of0.08mg/ml by intravenous injection,0.2ml per mouse and once six hour. Mice in Qingyitang were given by gavage with Qingyitang,0.5ml per mouse and oncesix hour. Mice in drug combination group were given equivalent medicines at the sameway mentioned above. Mice in blank control group and model group were givenequivalent normal saline by intravenous injection or gavage.Mice had been observed for7days on the basis of equivalent medicine intervention. After intervention treatment,mice were killed by cervical dislocation, terminal ileum, ileocecal junction and right halfcolon part were obtained sterilely for further study, hematoxylin-eosin staining wereperformed to the intestinal structures, colony counting value(CFU) were observed,comments were given based on pathology analysis, apoptosis Bax and apoptosis geneBcl-2were detected by Tunel method and immunohistochemistry technology, resultswere analyzed quantitatively by image analysis system.All datas were recorded by x±Sand analyzed by SPSS18.0statistics software.Results1. The modle success rate is86.25%.2. General conditions: Mice in blank control group were in good shape, lively andactive, their fur grew shiny. Mice in model group were dull and inactive, diet intakereduced, their fur was messy and not shiny. Mice in treatment groups were in good shapeand active a bit, fur grew shiny. Enterocoelia were opened after anesthesia anddisinfection, intestinal structure of mice in blank control group had no obviousabnormality, tissues were rosy, peristalsis worked smoothly. While mice in model groupwere found with congestion and edema, even necrosis in intestinal canal, enteric cavitywere flatulent and with dark red hydrops. Mice in treatment groups were found withcomparatively rosy intestinal canal, slightly congestion and edema, and flatulencedisappeared.3. Colony counting（CFU） of intestinal candida albicans: Intestinal candidaalbicans grew variously in blank control group, model group, somastostatin group,Qingyitang group and drug combination group, emerald smooth colony was found onchromagar salmonella medium. Colony counting in model group increased obviouslycomparing with blank control group（P<0.05）,after medicine intervention, colony counting in treatment groups dropped in varying degree, values had tremendousdifferenc（eP<0.05）, and difference of drug combination group ranked the firs（tP<0.05）.4. Pathological inspection and pathology comments: Mice’s intestinal and glandstructures in blank control group were inspected by optical microscope, inspection resultswere structures arranged orderly and completely, blood capillary in mucosa andsubmucosa had no congestion, glands had no swelling, inflammatory cells aggregated ordistributed dispersedly. As for mice in model group, results revealed that subepithelialclearance enlarged, mucosa and submucosa had congestion and swelling, glands arrangeddisorderly, blood capillary and tiny veins expanded and congested, intestinal mucosawere with necrosis and exfoliated, visible inflammatory cells aggregated and infiltrated.It has been confirmed by pathological analysis that these two groups had obviousdifference（P<0.05）.After medicine intervention, mucosa layer, submucosa and gapbetween lamina propria decreased, glands arranged orderly, blood vessel expanded just abit, exfoliation and necrosis reduced, a few of inflammatory cells can be found inintestine, treatment results of drug combination group ranked first based on pathologyanalysis（P<0.05）.5. Apoptosis detection and its index of intestinal mucosa cell: intestinal mucosaapoptosis detection was preformed on cell nuclei area, they were positive cells. A smallmount of apoptosis were detected in blank control group, as for model group, the volumeof mucosa cells reduced and distorted, intercellular space enlarged, cytoplasmconcentrated and cell membrane cracked into blocks, in severe case, apoptosis cells withapoptosis body took shape, which were claybank or yellowish-brown in cell nucleus.After medicine intervention, the number of apoptosis decreased variously in intestinalpathology analysis, remarkable difference had been found by comparing with modelgroup（P<0.0001）,difference of drug combination group ranked first（P<0.05）.6. Intestinal mucosa apoptosis gene Bax: Bax expressed on thick liquid area andwere distributed in mucosa cellular layer and glands, they were claybank positive cellsand distributed dispersedly sometimes. Comparing with blank control group, Baxexpression in model group has significanc（eP<0.05）.Bax expresses has some differencesin varying degree both in treatment groups and model group, there is no significance（P ﹥0.05）7. Intestinal mucosa apoptosis gene Bcl-2: Bcl-2expressed on thick liquid area andwere distributed in mucosa cellular layer and glands, they were claybank positive cells.Comparing with blank control group, Bax expression in model group has significance（P<0.05）.Bax expresses has some differences in varying degree both in treatmentgroups and model group, there is the most significance in combining Somatostatin andQingyi Somatostatin and Qingyitang（P<0.05）.Conclusions1、 Somatostatin and Qingyitang can reduce the number of candida albicans inmice’s intestinal canal, apoptosis decreases and drug combination provides acertain protection for intestinal mucosal barrier.2、 Somatostatin and Qingyitang could be the most effective and have aprotective role for intestinal mucosal barrier. |
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