| Objective: Intracranial infection is more common infectious diseases of acute of the central nervous system diseases in children.The pathogen invades the central nervous system can cause a series of pathological changes, they cause the different degree injury of brain tissue. In recent years, the biochemical signs of nervous system damage become one of the great attention of medical community.To observate levels change of PCT, NSE, CK-BB, LDH in cerebrospinal fluid and serum of acute and recovery phase children with intracranial infection, and to investigate the significance of differential diagnosis.Methods: We collected 64 children hospitalized in Cangzhou Central Hospital pediatric department from June 2013 to January 2015. The 64 children divided into 3 groups: purulent meningitis group 19 cases,viral encephalitis group 30 cases,normal control group 15 cases.All the cases after admission were collected venous blood and cerebrospinal fluid within 24 hours(incidence of 3 days), children with intracranial infection require were collected venous blood and cerebrospinal fluid again after hospitalization and medical treatment with 7 to 10 days. We collected the supernatant in a-70℃ refrigerator to batch determine the content of PCT, NSE, CK-BB and LDH in cerebrospinal fluid and serum using ELISA Kit which provided by Enzyme Biotechnology Limited Company, shanghai.Using SPSS19.0 statistical software analysis of experimental data. All measurement data were measured by mean±standard deviation(? ?s). Two samples mean were compared with T Test,The q test was used to pairwise diversity,Correlation between two variables judged by linear correlation analysis,P<0.05 as for that the difference was significant between them.Results:1 CSF data of children with acute phase were as follows:①The level of PCT: Purulent meningitis(PM) group was 0.84±0.40ng/ml, Viral encephalitis(VE) group was 0.25±0.13ng/ml, the control group was 0.21±0.11ng/ml,PM group compared with the control group and VE group,P<0.01, the difference was statistically significant,Comparison of VE group and the control group, P>0.05, there was no significant difference.②The level of NSE: PM group was 12.52±1.91μg/L, VE group was 14.36±2.82μg/L, the control group was 5.49±1.45μg/L, between the two groups, both P<0.05,the differences in each group were also statistically significant. The level of CK-BB: PM group was 15.46±4.72U/L, VE group was 17.77±2.93U/L, the control group was 4.40±0.78U/L, between the two groups, both P<0.05,the differences in each group were also statistically significant.④The level of LDH: PM group was 50.47±11.87U/L, VE group was 24.40±2.76U/L, the control group was 18.93±2.25U/L, between the two groups, both P<0.01,the differences in each group were also statistically significant.2 Serum data of children with acute phase were as follows:①The level of PCT: PM group was 10.13±5.43ng/ml, VE group was 0.52±0.31ng/ml, the control group was 0.48±0.33ng/ml,PM group compared with the control group and VE group,both P < 0.01, the difference was statistically significant; Comparison of VE group and the control group, P>0.05, there was no signifycant difference.②The level of NSE: PM group was 8.98±1.50μg/L, VE group was 10.75±2.56μg/L, the control group was 3.51±0.79μg/L, the differences in each group were also statistically significant.③The level of CK-BB: PM group was 6.52±1.46U/L, VE group was 8.25±3.26U/L, the control group was 3.08±0.60U/L, the differences in each group were also statistically significant.④The level of LDH: PM group was 342.63±80.92U/L, VE group was 369.70±112.64U/L, the control group was 274.80±42.88U/L, PM group and VE group compared with the control group,both P<0.01, the difference was statistically significant,PM group compared with VE group, P>0.05, there was no significant difference.3 The level of PCT, NSE, LDH in cerebrospinal fluid and serum of recovery phase children with PM group compared with VE group,P<0.01, the difference was statistically significant. The level of CK-BB in cerebrospinal fluid and serum of recovery phase children with PM group compared with VE group,P>0.05, there was no significant difference.4 The acute PM group level of PCT, NSE, CK-BB,LDH in cerebrospinal fluid compared with recovery phase children, P<0.01, the difference was statistically significant. The acute phase PM group level of PCT, NSE, CK-BB,LDH in serum compared with recovery phase children, P<0.01, the difference was statistically significant.5 The acute phase VE group level of PCT in cerebrospinal fluid and serum compared with recovery phase children, both P>0.05, there was no signifycant difference. The acute phase VE group level of NSE, CK-BB,LDH in cerebrospinal fluid compared with recovery phase children, all P<0.01, the difference was statistically significant. The acute phase PM group level of PCT, NSE, LDH in serum compared with recovery phase children, all P<0.01, the difference was statistically significant.6 There was a positive correlation between the cerebrospinal fluid and serum with acute PM children in the level of PCT(r=0.856,P<0.01),there was a positive correlation between the cerebrospinal fluid and serum with acute PM children in the level of NSE(r=0.968,P<0.01), there was a positive correlation between the cerebrospinal fluid and serum with acute PM children in the level of CK-BB(r=0.907,P<0.01), there was no correlation between the cerebrospinal fluid and serum with acute PM children in the level of LDH(r=0.369, P>0.05).7 There was a positive correlation between the cerebrospinal fluid and serum with acute VE children in the level of PCT(r=0.879,P<0.01), there was a positive correlation between the cerebrospinal fluid and serum with acute VE children in the level of NSE(r=0.979,P<0.01),there was a positive correlation between the cerebrospinal fluid and serum with acute VE children in the level of CK-BB(r=0.960,P<0.01),there was no correlation between the cerebrospinal fluid and serum with acute VE children in LDH level(r=-0.206, P>0.05).Conclusions:1 The level detection of PCT in cerebrospinal fluid and serum and the detection of LDH level in cerebrospinal fluid helps the diagnosis of purulent meningitis in the early stage of intracranial infection. The level detection of NSE, CK-BB in cerebrospinal fluid and serum helps the diagnosis of Viral encephalitis. Combined detection of four indexes conducive to the differential diagnosis for purulent meningitis and viral encephalitis.2 The dynamic observations of the level of PCT, NSE, CK-BB,LDH has important significance for the value condition monitoring and prognosis of children with intracranial infection.3 There was a positive correlation between the cerebrospinal fluid and serum with intracranial infection children in the level of PCT, NSE, CK-BB. Serum is looking forward to replace properly the cerebrospinal fluid detection, reduce the operation risk and the suffering of children, so that parents are more likely to accept. |
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