| Objective:The mechanism of allergic rhinitis was complex, numerous cells and cell factors took part in the process of the disease. Many scholars deemed the occurrence of AR as it was associated with the unbalance of Th1 /Th2 cells, but there still something be insufficient.In recent years,the study found that the regulatory T cells(Treg),the helper T cells(Th17) and the related cytokines IL-17 is closely related to the incidence of AR.The increasing attention of Treg/Th17 cell unbalance theory may be seen as an important supplement of Th1 /Th2 cell unbalance theory and of great significance on pathogenesis research of AR. In recent years, with the discovery and deep study of IL-35, scholars came to realize the significant role of IL-35 in immune response. As a kind of inhibitory cytokines, IL-35 had an effect on the differentiation and function of T cell subset and was important for the maintaining of Treg/Th17 cell unbalance. It had been proved that the quantitative and functional deficiency of IL-35 involved in the development of many diseases. However, the role of IL-35 in the pathogenesis of AR was still in deficiency at present, here, we planned to study it by detecting the expression level of IL-35, IL-17 and Treg in peripheral blood of AR patients and to research the role of them in the pathogenesis of AR, there by exploring the regulatory effect of IL-35 on the balance of Treg/Th17 cells and its value in immune treating of AR.Methods:In this study, 30 cases were randomly selected from outpatients of otolaryngological department in the second hospital of Hebei Medical university who were diagnosed AR, as a control group another 20 cases were healthy subjects enrolled from physical examination branch of our hospital, all cases met grouping criteria. 4ml peripheral blood was collected from the subjects, centrifuged as required, put the serum of upper layer into two tubes, detected the expression level of IL-35 and IL-17 in peripheral blood using double antibodies sandwich ELISA and the calculated concentration of IL-35 and IL-17 according to OD value that had obtained. Contemporaneously, 2 ml peripheral blood of subjects was collected and CD4+CD25+Foxp3+ T cell expression level was defeced via flow cytometry, the results were showed by the percentage of CD4+CD25+Foxp3+ T cells in CD4+ T cell. All experimental data was statistically analysized with SPSS19.0 software.All data in this study belonged to the measurement data and testified as normal distribution via normality test data, AR group and the control group were compared with two independent samples T-test, correlation analysis was made using Pearson correlation test,all statistics were double side probability test,α= 0.05,P<0.05 was considered statistically significant.Results:1 Compared between the two groups, the expression of IL-35 was 54.127±8.138 pg/m L in AR group and 71.622± 6.227 pg/m L in control group, the expression level of IL-35 in AR group was obviously lower than that in control group, the difference was a statistically significance(t=-8.145,P<0.01).2 Compared between the two groups, the expression of IL-17 was 422.267±55.140 pg/m L in AR group and 261.103±17.070 pg/m L in control group, the expression level of IL-17 in AR group was obviously higher than that in control group, the difference was a statistically significance(t=14.969,P<0.01).3 There was a remarkable negative correlation between the IL-35 and IL-17 expression in the serum of AR group(r=-0.773,P<0.01).4 The percentage of CD4+CD25+Foxp3+ T cells in CD4+ T cell was 3.917±0.820 in AR group and 6.861±0.616 in control group, the percentage of CD4+CD25+Foxp3+ T cell in CD4+ T cell was significant lower in AR group than that in control group(t=-13.678,P<0.01).Illustrated the differences in the CD4+CD25+Foxp3+ Treg expression between AR group and the control group,with significant difference statistically.5 Correlation analysis between the percentage of CD4+CD25+Foxp3+ T cell in CD4+ T cell and serum IL-35 level was made within AR group and the result showed there being a positive correlation between the two(r=0.736,p<0.01).6 Correlation analysis between the percentage of CD4+CD25+Foxp3+ T cell in CD4+ T cell and serum IL-17 level was made within AR group and the result showed there being a negative correlation between the two(r=-0.630,p<0.01).Conclusions:1 Serum IL-35 expression level in AR group was obviously lower than that in control group, while Serum IL-17 expression level in AR group was obviously higher than that in control group, which indicated that decreased secretion of IL-35 and increased secretion of IL-17 were related to the incidence of AR.2 In AR group, there was a remarkable negative correlation between serum IL-35 level and serum IL-17 level, IL-35 was a kind of important inhibitory cytokine in immune response, the incidence of AR may be correlate with the hyperfunction of Th17 cell and the increased secretion of IL-17 caused by weakened IL-35 mediated immunosuppressive effects.3 The percentage of CD4+CD25+Foxp3+ T cell in CD4+ T cell was lower in AR group than that in control group, which indicated that the differentiation and function of Treg cell in AR group were suppressed and this may be the important mechanism for the occurrence of AR.4 There being a positive correlation between the percentage of serum IL-35 level and CD4+CD25+Foxp3+ Treg cell in CD4+ T cell in AR group, that was, both the IL-35 and CD4+CD25+Foxp3+ T cell level in peripheral blood decreased in AR group, indicated that declined expression of Treg cell may decrease the secretion of IL-35 or decreased secretion of IL-35 weakened its promotion effect on Treg cells, there being interactions in the occurrence of AR.5 There being a negative correlation between the percentage of serum IL-17 level and CD4+CD25+Foxp3+ Treg cell in CD4+ T cell in AR group and IL-17 were mainly secreted by Th17 cells, which indirectly indicated that there may exist hyper functional Th17 cells in peripheral blood and Treg / Th17 unbalance mechanism in AR group.6 This experiment indirectly proved that IL-35 was important cytokines that affected the incidence of AR, decreased expression of IL-35 may inhibit the proliferation of Treg cells, lead to hyper function of Th17 cells, increase secretion of s IL-17 and result in unbalance of Treg / Th17 cells; these may be the important mechanism of the occurrence of AR, regulation of IL-35 may become a new target for the immunological therapy of AR. |
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