Study On The Correlation Between The Expression Of VEGF And Its Receptors In Psoriasis Vulgaris And TCM Syndrome Type

Objective:①Through the detection of the lesions of patients with psoriasis vulgaris in vascular endothelial growth factor and its receptors VEGFR-1(Flt-1),VEGFR-2(KDR)expression,analyze their differences between in the lesions of patients with psoriasis vulgaris and in normal skin,and theie correlation with psoriasis;and using CD34 labeled vascular endothelial cell,count microvessel density(MVD) in order to better understand the relationship between angiogenesis and psoriasis.②By testing serum level of VEGF of the different Chinese medicine dialectical typing of patients with psoriasis vulgaris,to investigate the association of different TCM syndrome types of psoriasis and VEGF pathway.Subjects and methods:①The pathological specimens:patients with psoriasis vulgaris skin specimens from January 2014 to January 2015 in shaanxi province Chinese medicine hospital dermatology clinic patients,a total of 20 cases,and exclude other systemic diseases.All patients were confirmed by clinical and histopathological examination confirmed the diagnosis of psoriasis vulgaris,and in the progress of.Normal skin tissue from Miao Hou Street community hospital outpatient operating room after circumcision foreskin tissue,a total of 10 cases.By immunohistochemical SABC method,detecting the experssion and location of VEGF,FLT-1,KDR,CD34 in skin lesions and the lesions of psoriasis vulgaris.Using semi-quantitative scoring method,analysis the differences of the expression level and significance between lesion group and normal control group.②Serum samples:the serum samples of patients with psoriasis vulgaris from Shaanxi Province Chinese Medicine Hospital Department of Dermatology Hospital patients,a total of 50 cases,including 20 cases of blood heat syndrome,10 cases of blood dryness syndrome,20 cases of blood stasis,and exclude other systemic diseases.Using double antibody sandwich enzyme linked immunosorbent assay for determination of serum VEGF expression level,according to the results of analysis of the differences between the normal control group with psoriasis group,carries on the comparison to Chinese medicine dialectical type three group analysis.③The analysis of the experimental result:simmunohistochemistry experiment results using the independent samplest test,Mann-whitney U test and Pearson rank correlation analysis in SPSS19.0 statistical software.The experimental results of ELISA by single factor variance analysis in SPSS19.0 statistical software.To P<0.05,P<0.01 was considered as significant boundaries.Results:①Detected in the lesions of patients with psoriasis vulgaris an positive expression level of VEGF was obviously higher than that of normal control group,the difference was statistically significant(P<0.05).②The positive expression of KDR and FLT-1 in psoriasis vulgaris patients were higher than those in normal control group,and there was significant difference(P<0.05).Among them,the KDR strong positive expression in the lesion group was 55%,the FLT-1 strong positive expression in the lesion group was 45%.③In normal skin and psoriatic lesions,MVD values were normal control group=(7.52±1.54),lesions group=(13.86±4.29),compared to MVD value of two,with statistical difference(P<0.05).④THE expression of VEGF,FLT-1,KDR and MVD in psoriasis vulgaris,the pairwise comparison,showed a positive correlation with statistical significance(P<0.01,P<0.05).⑤Of traditional Chinese medicine of three groups of patients serum VEGF levels are higher than normal control group,P<0.05,with statistical significance.The serum VEGF level of TCM syndrome differentiation in patients of the three groupswere compared with each other,blood heat syndrome group>blood dryness syndromegroup>blood stasis group,compared to Blood heat syndrome group and blood dryness syndrome group, blood stasis group was statistically significant(P<0.05),compared to blood dryness syndrome group and blood stasis group is no statistical significance(P<0.05).Conclusion:In the pathogenesis of psoriasis,There is a close relationship between abnormal angiogenesis and the occurrence,development and recurrence of psoriasis vulgaris.Therefore,the neovascular formation helps to reveal the pathogenesis of the psoriasis,guiding clinical therapy.Our results show that,detection of expression of VEGF and its receptor FLT-1,KDR and CD34 in the lesions can clear the importance of microvessel formation in the pathogenesis of psoriasis,and provide the theoretical basis to identify new therapeutic targets.We also by detecting the VEGF level of serum of different TCM syndrome types with psoriasis vulgaris patients,discussed the relationbetween the different TCM Syndromes of psoriasis and VEGF.For modernization of traditional Chinese medicine research provides an important theoretical basis.