Comparative Study On Pharmaeoresistant Epileptic Rat Model Estabilshed By Two Excperimentize Methods

Objective:In this paper, the methods of fast electrical amygdaloid kindling or pilocarpine intraperitoneal injection were uesd to establish the epileptic rat model and then,the traditional and epileptic drags were used to select out the drag-resistant epilptic rats.A comparative study was parformed on behavioral and EEG changes in these model.Methods:(1)Establishment of the model of epilepsy: Forty adult male SD rats,weighing 223.5±1.3g,were ramdomized to two groups,each included 20 rats. In group A,the epileptic rat model was estcplished by amygdaloid kindling,the parameters inctuded the following:stimulating frequency was 16 Hz, the wave width was 1.0ms,the stimatation,intensity was 0.5m A intensity and the string length was 10. In group B, the rats were injected intraperitoneally with lithium chloride and the pilocarpine to prepare the epileptic model. The successfully prepared epileptic rat model were used in the next experiment.(2)Selection of drug-resistant epileptic rats:The two traditional onci-epileptic drugs were used to select the pharmaeoresistance. Firstly, the carbamazepine was uesd to select the pharmacoresistance, then the sodium valproate was followed. After the epileptic rats expertenced the two drugs selection,the spontaneously epileptic behavior remained more than 10 times a week,these rats were considered as the drug-resistant epileptic model.(3) The behaviors of the two pharmeo resistant epileptic rats were observed. The EEG data of each epileptic rat were collected to conduct a statistical analysis.Results: Fourteen rats were successfully kindeled in amygdaloid group,with a 50% of successful kindeling rate.in pilocarpine group,17 epileptic models were established,with a successeful rate of 75%. The difference between the two groups was statistically significant. The in success the the pilocarpine group was higher thanthat of the fast amygdaloid kindled group(P=0.043);(2)Spontaneous recurrent attacks from I to V degree were observed in the epileptic model estabished by the two methods.There is no statistical significance in the seizure degrees between the two groups(P=0.036);(3) The average time of the epileptic seizures in amygdaloid kindeling pharmac resistant epileptic rats was 21.6±5.94 s. While, in the pilocarpine-induced model, the average time of the epileptic seizures was 53.0±14.83 s. A sigmficont difference in the seizure duration was noted between the two groups(T=4.39, P=0.002).Conclusion: Compared with the amygdaloid kindled model, the successful rate of the epileptic model induced by pilocarpine was increased. The epileptic behavior of the epileptic animal model established by pilocarpine could last a longer time, which could be used clinically.