Expression And Clinincal Significance Of Human Tolemerase Gene In Cervical Tissue With Persistent Infection Of Human Papilloma Virus

Cervical cancer is the second most common gynecologic malignancy inthe world. In recent years, the incidence and fatality of cervical cancer havefallen significantly, due to the work of cervical cancer screening. It iswidely recognized that cervical cancer is caused primarily by persistentinfection with high-risk human papillomavirus. In approximately70%ofcervical carcinomas, the most consistent aberration is gain of chromosomearm3q, including hTERC. The study is to explore the expression andclinincal significance of human tolemerase gene in cervical tissue withpersistent infection of human papilloma virus.The study population included women with HR-HPV who attendedcervical cancer screening worked by The First Affiliated Hospital of HebeiNorth University gynaecoloyg＆obstetrics department in HebeiChicheng. After8-12months, they accepted Cervista HPV HR test again,from which we selected randomly70positive cases as experimental groupand20negative cases as control group. This study used Cervista HPV HRtest to detect the infection of14HR-HPV subtypes in cervical exfoliatedcells. Positive cases are divided into three groups: groupA5/A6(51，56，66),groupA7(18，39，45，59，68), groupA9(16，31，33，35，52，58). Experimentalgoup included20cases of A9,15cases of A7,15cases of A5/A6and20cases mixed infection group. All subjects underwent colposcopicexamination and colpscopically multiple biopsy with their consent andknowledge. hTERC gene in cervical tissue samples was determined byusing fluorescence in site hybridization. Positive expression rates of hTERC gene in negative group、A5/A6，A7，A9，mixed infection group were5%，33.33%，53.33%，85%，50%.There was statistical difference between A9and other groups(P＜0.05).Positive expression rates of hTERC gene in cervical normal tissue＆inflammation group，CIN1，CIN2，CIN3were13.79%，33.33%，66.67%，93.75%. There was statistical difference between CIN2/3and othergroups(P＜0.05).hTERC amplification is related to the subtypes of HPV infection,especially A9. hTERC gene amplification showed a trend of increasing,with the progress of the cervical lesions. hTERC gene has definite clinicalsignificance for diagnosis of cervical lesion grade and predict the progressof the cervical lesion.