Investigation Of The Association Between HLA-DQB1,HLA-DRB1Genetic Polymorphism And Kidney Deficiency Syndrome Of Chronic Hepatitis B~*

ObjectiveTo investigate the HLA-DQB1, DRB1genes polymorphism of kidney deficiency syndrome of Chronic Hepatitis B, and to study the relationship between polymorphisms of HLA-DQB1alleles and kidney deficiency syndrome of Chronic Hepatitis B, to provide cluesto seeking for the susceptible or antagonistic genes, and explore the biologic mechanism that pathogens accumulated affect HBV persistent infection.MethodsRandomly select kidney-yang deficiency patients, kidney-yin deficiency patients, asymptomatic patientsand healthy volunteers, apply PCR-SBT to detect HLA-DQB1alleles and HLA-DRB1alleles; and collect HBV-DNA,HBV markers, ALT, AST, TBIL DBIL. Apply SPSS software to analyze the correlation among alleles and CHB kidney deficiency syndrome, laboratory index. Count data are tested through chi-square test.Measurement data results are represented by X±S; two sets of measurement data are tested through independent samples t test.Results:1.13DQB1alleles and20DQB1alleles were detected in120blood samples; In the chronic hepatitis B group, DQB1*0607, DRB1*0401were not detected. DQB1*030101G, DQB1*0303,DQB1*0601/02,DRB1*0901,DRB1*1202were the common alleles,and the alleles frequency were21.21%,22.73%,17.68%,21.72%,12.63%. In the normal control group, DQB1*0302, DQB1*0610, DRB1*0405/06, DRB1*1001, DRB1*1602were not detected, DQB1*030101G. DQB1*0303, DQB1*0502,DRB1*0901were the common alleles, and the alleles frequency were30.95%、23.81%、9.52%,23.81%。The positive rate of DQB1*0601/02inthe chronic hepatitis B group was17.68%, were higher than4.76%in the normal control group(P<0.05)。2.The positive rate of DQB1*0601/02inthe chronic hepatitis B group was17.68%, was higher than4.76%in the normal control group(P<0.05, OR=4.30,95%CI:1.00-18.61); The positive rate of DQB1*0601/02in the asymptomatic group was19.57%, was higher than4.76%in the normal control group(P<0.O5,OR=4.87,95%CI:1.07-22.04); The positive rate of DRB1*120101G in the asymptomatic group was0.00%,was lower than7.14%in the normal control group(P<0.05).The positive rate of DQBl*0601/02inthe kidney-yin deficiency group was18.00%, was higher than4.76%in the normal control group (P=0.051).3. The positive rate of DQB1*0201in the kidney-yang deficiency group was higher than the asymptomatic group(14.29%vs4.35%, P<0.05,OR=3.67,95%CI:1.05-12.81).The positive rate ofDRB1*0301in the kidney-yang deficiency group was higher than the asymptomatic group(14.29%vs4.35%, P<0.05,OR=3.67,95%CI:1.05-12.81).The positive rate of DQB1*0201/02in the kidney-yang deficiency group was higher than thekidney-yin deficiency group (17.85%vs4.00%, P<0.05, OR=5.22,95%CI:1.08-25.11).4. There was no significant difference in HLA-DQB1alleles and DRB1alleles positive rates between kidney-yang deficiency group and normal control group (P>0.05). There was no significant difference in HLA-DQB1alleles and DRB1alleles positive rates between kidney-yang deficiency group and normal control group(P>0.05).5. The positive rate of DQB1*0303in the HBV-DNAnegative group was17.50%, was lower than40.48%in the low replication group(40.48%, P<0.05, OR=0.31,95%CI:0.13-0.73. And18.42%in thehigh replication group, was alsowas lower than40.48%in the low replication group(40.48%, P<0.05, OR=0.33,95%CI:0.14-0.77).The positive rate of DRB1*0901in the HBV-DNA negative group wasl6.25%, was lower than40.48%in the low replication group (40.48%, P<0.05, OR=0.29,95%CI:0.12-0.67).And17.11%in thehigh replication group, was alsowas lower than40.48%in the low replication group (40.48%, P<0.05, OR=0.30,95%CI:0.13-0.72).The positive rate of DRB1*12O2in the HBV-DNA negative group was18.75%,13.16%in thehigh replication group, were higher than in the low replication group(0.00%,P<0.05). Conclusions:1. DQB1*030101G, DQB1*0303, DQB1*0601/02, DRB1*0901, DRB1*12O2were the common alleles. In the chronic hepatitis B patients,and in the healthy people, DQB1*030101G, DQB1*0303, DQB1*0502, DRB1*0901were the common alleles。2. DQB1*0601/02may be the susceptible genes of chronic hepatitis B in Sichuan Provinceo3. DQB1*O2O1, DRB1*O3O1may be the susceptible genes of kidney-yang deficiency syndrome.4. HLA-DQB1/DRB1were closely related to the viral loads.The chronic hepatitis B patients with DQB1*0303、DRB1*0901may had a low viral reconstructed active level.