The Effect Of Passive Smoking On The Bone Mineral Density And Bone Metabolism Of Castrated Female Rats

Objective:People mistakenly hold the idea that the damage of smoking is only within thesmokers themselves, which is very reverse of the truth. Because not all of the smokeis breathed directly into the body of smokers, so many people think that the carbondioxide breathed out is breathed in by people around. The harm of passive smoking topeople is thought to be emerging, especially to the women who stop menstruating.Passive smoking may accelerate bone metabolism and make women who stopmenstruating suffer from OP ahead of time. This research aims to provide theory basisby adapting an animal experiment.Methods:40wister rats were distributed randomly into4groups: A, C, B andD. Group A:pseudo-operation; Group B: castrated rat; Group C: passive smoking; Group D:castrated rat and passive smoking. Metabolize biochemical indicator of wister rats ismeasured after being testing for one, two, three months for these four groups: CA, CRand the ratio of Ca/Cr, serum osteocalcin. Then the bone density is measured and thecomparison between these four group is made.Results:One month after experiment: there is no difference of the rats’ bone density, boneglaprotein and the ratio of Ca/Cr between Group A, Group C and Group B (p>0.05).There are some differences of of the bone mineral density, bone glaprotein and theratio of Ca/Cr between Group D and Group A, Group B and Group C (p<0.05).Twomonths after experiment: there is some differences of bone mineral density betweenGroup B and Group D（p<0.05）. There are some differences of the bone mineraldensity, bone glaprotein and the ratio of Ca/Cr between Group B and Group A, GroupC (p<0.05).There are some differences of the bone mineral density, bone glaproteinand the ratio of Ca/Cr between Group D and Group A, Group C.(p<0.05). Three months after experiment: The groupB、D have distinctive differences of the bonedensity, bone glaprotein and the ratio of Ca/Cr (p<0.05); the differences of the bonemineral density, bone glaprotein and the ratio of Ca/Cr of Group B is larger than thatof Group D.Conclusion:1.Castrated rat have changes in bone metabolism and bone density earlier if givenpassive smoking than they are castrated or given passive smoking respectively, so thebiochemical indicator of bone mineral density and bone metabolism of castrated ratsare more susceptible to passive smoking. Castrated rats which are given passivesmoking are more likely to suffer from OP earlier.2. OP after post-menopause iscorrelated with passive smoking called dose-effect relativity. Castrated rats which aregiven passive smoking suffer from more severer OP than those are not given passivesmoking.