Effects Of Artichoke (Cynara Scolymus L.) Leaf Extract On Alcohol-induced Injury Of HepG2Cell

Objective:The method was developed to determine the functional component of artichoke leaf by HPLC, and investigate the effect of Artichoke(Cynara scolymus L.) leaf extract on alcohol-induced HepG2cell damageMethods:A method was developed to determine chlorogenic acid and cynarin in artichoke(Cynara scolymus L.) by HPLC. AECOSIL C18(4.6mm×200mm,5um) column was used with the mobile phase of0.2%phosphoric acid water solution and acetonitrile solution, the flow rate was at1.OmL/min, the wavelength was330nm, column temperature was29℃. HepG2cellular injury models induced by alcohol was eatablished to investigate the effect of Artichoke(Cynara scolymus L.) leaf extract on alcohol-induced HepG2cell damage. Alohol-induced HepG2cell damage model was established by using alcohol added to the cell culture medium of HepG2cell and using hepatoma cell line HpeG2as experimental materia. MTT assay was used to screen out the alcohol concentration, time and the optimal concentration of Artichoke leaf extract in this reearch. The effect of Artichoke leaf extract on alcohol-induced HepG2cell damage was evaluatd by detecting the activity of intracellular TG,GSH and SOD, the content of ALT, AST and MDA in each group cell culture medium,and through the cell RNA extraction, detection of PPARy mRNA and TNF-a mRNA expressionResults:This method showed a good linearity between peak area and concentration of injection.The results demonstrated high accuracy and stability of this method.And the research showed that AST, ALT, TG and MDA levels were significantly decreased,GSH and SOD activity increased significantly, compared with model group, when HepG2cell culture medium were treated with0.6%,1.2%,2.4%alcohol concentration joint with2.5ug/ml,5ug/ml, lOug/ml Artichoke leaf extract by48h,and the PPARy mRNA expression quantity to increase, the TNF-a mRNA expression quantity reduced, effectively reduce the alcoholic liver damage produced by the expression of related genes. The protective effect of Artichoke leaf extract on alcohol-induced HepG2cells damage was enhanced with the cocentration of the extract used.Conclusion:Artichoke extract on alcohol induced HepG2cell injury has a protective effect. The mechanism may be related to lower lipid peroxidation damage degree, removal of oxygen free radicals, enhance the vitality of antioxidant enzymes,and reduce the alcoholic liver damage produced by the expression of related genes.This experiment for application of artichokes in prevention and mitigation provides theory basis for alcoholic liver damage.