| Objective: This topic is to observe the effects of Qianggan Ruanjian Pill on rat liver fibrosis in general, morphological changes in liver and the expression of MMP-2, TIMP-2 in liver tissue use animal experimental study. Objectively the effect of Qianggan Ruanjian Pill on hepatic fibrosis and the intervention and treatment, and to probe its mechanism, develop ideas to further promote the application of traditional Chinese medicine on clinical.Methods: 120 SD male rats were selected in this experiment, weight of 180-200 g. Randomly divided into 6 groups:Normal control group, Model control group, Colchicine intervention group, Colchicine treatment group, Qianggan Ruanjian Pill intervention group, Qianggan Ruanjian Pill treatment group. Except the normal control group, each group use uninactivated porcine serum intraperitoneal injection to manufacturing models, two times a week, each time each one 0.5ml, 10 weeks. Normal control group with the same method, the same dose, with the of intraperitoneal injection of normal saline. The intervention group was intragastricing administration at the same time intraperitoneally injecting, one time a day, with colchicine solution: 0.1g/Kg(colchicine concentration 0.01g/ml), Qianggan Ruanjian Pill solution 2g/Kg(drug concentration 0.2g/ml). The normal group, the model group and the treatment group with the same dose of sterile water gavage every day. At the end of the fifth week, each group will select 5% rats randomly and kill them by decapitation method, then liver pathology will prove that we make model successed. At the beginning of sixth week, each treatment group will given intragastric administration of drugs, and the same dose in each intervention group. Each group selected ten rats randomly and decapitated at the end of the tenth week. Methods routine HE staining and Masson staining to observe the morphological changes of liver tissue in rats of each group by cytology; immunohistochemical staining, in liver tissue were detected by MMP-2 in each group of matrix metalloproteinase and its inhibitor TIMP-2 using image analysis.Results :(1)Qianggan Ruanjian Pills can improve the immune hepatic fibrosis in rats on the degree of liver fibrosis and in general.(2) Qianggan Ruanjian Pills can rise the expression of MMP-2, reduce the expression of TIMP-2 in rats with liver fibrosis, and inhibition of ECM synthesis and promote its degradation, and inhibit the formation of liver fibrosis.Conclusion: Druing the experimentment use, Qianggan Ruanjian Pill can obviously improve the general situation in rat with liver fibrosis, rising the expression of MMP-2 and reducing TIMP-2 in liver tissue, reducing the degree of liver fibrosis and inhibiting the development of hepatic fibrosis. |
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