Radiation Crosslinked Biobased Composite Hydrogel For The Study On Promoting Wound Debridement And Healing

Debridement was a key step in the preparation process of wound bed. Clinically, to protect all kinds of wounds caused by burn, trauma, operation, infection, removal foreign body of wound and necrotic tissue, control wound infection, maintain the wound moist environment, a variety of different tools and treatment products were used, however most of existing treatment methods and therapeutic effect were unsatisfactory.Polymer materials were selected in this paper which combined the advantages of these materials and make up the shortage of single polymer material, played in the debridement, wound healing and other aspects of the function. The hydrogel was prepared using a variety of natural polymer, which form was paste and extrusion. The paste shape made it applicable to different extent (the skin deep region that diaphragm or patch cannot touch) and the wound of irregular shape. The hydrogel could fill and contact wound with full range and no dead angle, play its debridement and wound healing function.The fitting materials and its derivatives were select in this paper, for the preparation of hydrogel by the radiation cross-linking method. Due to no toxic chemical crosslinking agent in the preparation process which abandon the traditional process. The hydrogel prepared by the radiation cross-linking method which had good biocompatibility and stable structure. The radiation crosslinking technology both had the irradiation and sterilization effect. To a certain extent, which could reduce the complexity of the process and enhance its maneuverability, could be large-scale production. According the domestic and foreign standards, the structure characterizes and the performance of the paste material was tested. The swelling ratio, degradation rate, compressive strength, pore structure, thermal stability and other properties all had certain regularity with the ratio of raw materials and irradiation conditions. The results showed the material had the good physical and mechanical properties.The cytotoxic of hydrogel materials were investigated by L929 fibroblasts in this paper, the results show that debridement materials had no obvious effect on the proliferation of L929 cells and the cell toxicity grade was 0 or 1 that the material had good cell compatibility. Meantime, the necrotic tissue scald model and full-thickness skin wound models were built and hydrogel could remove necrotic tissue and promote wound healing. Experimental results showed the hydrogel material could maintain a moist healing environment and inhibit infection. Meantime, hydrogel had good mechanical properties and high swelling ratio. Hydrogel could remove necrotic tissue and promote wound healing effectively in animal experiments and promote the proliferation of fiber cell, neovascularization formed so that the wound healing rate was high. Hydrogel had good biological compatibility and effectiveness which laid the foundation for industrialization and clinical application in the future.