The Study Of The Relationship Between 8-iso-PGF2a,AOPP, SOD And CKD Patient

Objectives: To discuss the affecting factors and the relationship of 8-iso-PGF2 a,advanced oxidation protein products(AOPP), serum superoxide dismutase(SOD) of chronic kidney disease(CKD) patients. Exploring the relationship of serum creatinine(Scr) level, rate of glomerular filtration(GFR) with 8-iso-PGF2 a, AOPP,SOD, and the thyroid hormone level. For further research on connections between oxidative stress, nutritional status, disease progression and cardiovascular complications of patients with CKD. And to provide new clinical evidence and therapeutic strategy.Methods: According to the inclusion and exclusion criteria, our study randomly recruited the patients who were diagnosed CKD 1-5 stage and healthy controls in the Second Affiliated Hospital of Nanchang University from January 2014 to December2014. With the method of Sandwich ABC-ELISA we detected the blood sample of8-iso-PGF2 a, AOPP, SOD level and we collected the related clinical biochemical indicators in laboratory. We divided the volunteers into 4 groups based on the staging criterion of CKD. By using the SPSS19.0 software we analyzed the statistical data.Correlative factors in different groups are compared by variance analysis and using Pearson analysis or linear regression to analysis their relevance.Results: 1 、 Total of 404 objects were brought out to our study(374 CKD patients, 114 in CKD1-3 group, 98 in CKD 4 group, 132 in CKD 5 group and 30 in normal control group). We randomly selected 22 cases from each group. Through the experiments we obtained 88 blood specimen concentrations of 8-iso-PGF2 a, AOPP and SOD. The blood biochemical indexes and basic information were collected from laboratory. 2 、 There was a negative relationship between concentrations of8-iso-PGF2a(U/ml) with GFR(ml/min) in CKD patients. The correlation coefficient was-0.574(P < 0.01). The linear regression equation was Y=-1.9328X+1535.2(Y was the concentration of 8-iso-PGF2 a, X was the level of GFR). We only found that CKD5 was different with the normal control group(P < 0.01). GFR could influencedon the serum concentration of 8-iso-PGF2 a. The coefficient of determination was0.087. 3 、 By the statistical method of correlation analysis and linear regression analysis, we found that there was a negative relationship between concentrations of AOPP(umol/l) with GFR(ml/min) in CKD patients. The correlation coefficient was-0.504(P < 0.01). The linear regression equation was Y=-0.0879X+38.046(Y was the concentration of AOPP, X was the level of GFR). Through the method of variance analysis, we compared the difference of CKD groups and normal control group. The result suggested that there was a significant different between the normal control group and CKD groups(included CKD1-3 group, CKD 4 group and CKD 5group)(P < 0.01, P < 0.01, P < 0.01). There was no significant difference between CKD1-3 and CKD 4 group(P = 0.087). Significant difference was found between CKD 5 and CKD1-3 group(P < 0.01), however no difference between CKD 5 and CKD 4 group(P = 0.06). By using multiple linear regression analysis method, we found GFR, erythrocyte sedimentation rate could influenced on the serum concentration of AOPP. The coefficient of determination was 0.289. 4、We found that there was a positive relationship between concentrations of SOD(U/ml) with GFR(ml/min) in CKD patients. The correlation coefficient was 0.586(P < 0.01). The linear regression equation was Y=0.458X+116.32(Y was the concentration of SOD,X was the level of GFR). We found that CKD4 group and CKD5 group was different with the normal control group(P < 0.01, P < 0.01), however no difference between normal control group and CKD 1-3 group(P = 0.113). Significant difference was found between CKD 1-3 and CKD 5 group(P < 0.01), Difference was also found between CKD 4 and CKD 5 group. However no difference between CKD 1-3 and CKD 4 group(P = 0.217). Also, we found Hb, Scr could influenced on the serum concentration of SOD. The coefficient of determination was 0.322. 5、 We found there was a positive relationship between concentrations of FT3(pg/ml) with GFR(ml/min) in CKD patients. The correlation coefficient was 0.574(P < 0.01). The linear regression equation was Y=0.0091X+1.979(Y was the concentration of FT3,X was the level of GFR).The result suggested that the levels were significantly decreased in all groups of patients relative to the controls(P < 0.01、P < 0.01、P <0.01), and CKD4 group and CKD5 group was different with CKD1-3 group(P <0.01、P < 0.01). However no difference between CKD 5 and CKD 4 group(P =0.538). Hemoglobin, SOD, AOPP, Scr could influenced on the serum concentration of FT3. The coefficient of determination was 0.502.Conclusions: GFR of patients with CKD was negative with AOPP and8-iso-PGF2 a respectively and positive with SOD and FT3. There was significant correlation between level of oxidatie stress and FT3. Oxidatie stress was existed in patients with CKD, and as the GFR decline, the blood Scr and oxidative stress level were higher, FT3 level gradually reduced. There was a relationship between low FT3 level and oxidative stress. FT3 level was positive with SOD and negative with AOPP and 8-iso-PGF2 a.