Study On The Tumor Markers Of Hepatocirrhosis Patients Caused By HBV

ABSTRACTObjective: Active replication of hepatitis B virus is a risk factor for the occurrence and development of hepatocirrhosis, which can further developed to primary hepatocellular carcinoma(HCC). This study investigated the relationship between serum HBV-DNA loads and the content of tumor markers of hepatocirrhosis patients caused by HBV by combined analysis, in order to find the relationship between HBV-DNA loads and the development of hepatocirrhosis of hepatitis b patients and the risk of these patients to developed to HCC, and to provide theory foundation for antiviral treatment to decrease the risk of the development of hcc.Methods:1 General Information1.1 Liver cirrhosis group30 cases of patients aged from 18 to 65 years with hepatitis B cirrhosis,administrated from January 2011 to December 2013, in the Xingtai Third hospital and other two hospitals, were selected as liver cirrhosis group. All patients with hepatitis B cirrhosis were diagnosed according to the diagnosis standard, and patients with cirrhosis caused by other causes were ruled out. In the 30 cases of chronic hepatitis B patients with cirrhosis, 8 cases of them were Child-Pugh A, 12 cases B, 10 cases of Child-Pugh C according to Child-Pugh grading standard. 10 cases of patients with liver cirrhosis were in the compensatory stage and 20 cases of patients with liver cirrhosis decompensation stage according the national classification standard for lever function1.2 Hepatocellular cancer control group30 cases of patients of hepatocellular cancer diagnosed between January2011 and December 2013, in the Xingtai third hospital and other two hospitals,were selected as liver cancer control group, All of the patients were in line with the diagnostic criteria for hepatocellular carcinoma, aged from 18 to 65 years and patients with liver cancer caused by other causes were ruled out.1.3 Normal control group30 cases of healthy people of medical center in the Third hospital of Xingtai aged from 18 to 65 years, were selected as normal control group.1.4 Basic information of the three groupsThere were no statistically significant difference in gender, age, weight,nationalities, culture background among the subjects(P > 0.05).2 Research methods2.1 Collection of specimen5ml peripheral venous blood of the subjects fasting for 8 to 10 hours were collected, and standed for 2h at room temperature, then centrifuged 10 minutes at 3000 rpm/min to separate the serum and keeped at- 80 ℃.2.2 Detecting methodsHBV-DNA was detected by PCR method; AFP, CEA,AFP- L3, GP73 were detected by double antibody sandwich ELISA method.2.3 The test results were analyzed with SPSS 18.0 software. The correlation were analysed between HBV- DNA and AFP- L3, GP73, AFP, CEA indicators The Spearman test for HBV- DNA and AFP- L3, GP73, AFP, CEA indicators such as correlation analysis; Inspection standards as the alpha =0.05, P < 0.05 there was no statistically significant difference said.Results:1 The levels of serum GP73, AFP, AFP-L3, CEA of liver cancer control group were statistically higher than those of liver cirrhosis group and normal control group(P <0.01). The levels of serum GP73, AFP, AFP-L3, CEA of liver cirrhosis group were statistically higher than those normal control group(P < 0.01).2 The levels of serum GP73, AFP, AFP-L3, CEA of in patients with of5-6 log10copies/ml were significantly higher than those of the patients with HBV-DNA load 3-4 log10 copies/ml(P < 0.01).3 The levels of serum GP73, AFP, AFP- L3, CEA level were significant between the compensatory cirrhosis group and decompensated cirrhosis group(P<0.01); The levels of serum GP73, AFP, AFP- L3, CEA of decompensated cirrhosis group were significantly higher than those of compensatory cirrhosis group(P < 0.01).4 The levels of serum GP73, AFP, AFP- L3, CEA concentration in different Child-Pugh grad groups were analyzed. The results showed that,along with the increasing of Child- Pugh grading, the levels of serum GP73,AFP, AFP- L3, CEA concentration raised significantloy(P < 0.01).Conclusions:1 The results showed that the expression of GP73, AFP-L3, AFP, CEA and other indicators of liver cirrhosis patients increased with the increase of liver function, DNA HBV load was higher than those of GP73, AFP-L3, AFP and CEA。2 The detection of serum HBV-DNA, GP73, AFP, AFP-L3, CEA and in patients with hepatitis B cirrhosis may be early detection of liver cancer development trend, and timely clinical intervention,3 reduce GP73, AFP, AFP-L3, CEA, HBV-DNA and other indicators, so as to reduce the degree of liver function damage, delay the process of liver cirrhosis, reduce or postpone the occurrence of liver cancer.4 GP73, AFP, AFP-L3, CEA and other indicators increased, to a certain extent, reflect the activity of chronic hepatitis, liver function damage degree and the degree of liver cirrhosis. As chronic hepatitis activity, liver function and the degree of liver damage and the degree of cirrhosis of the liver of the non interventional detection index, may have a certain clinical application significance.