The Components Analysis And Research On Antibacterial Activity Of The Fibre Of Coptis Chinensis Franch

Coptis chinensis Franch.(FCC) belongs to renascent herbs and is harvested once every five years.It is deemed as a famous Chinese herbal medicine since ancient times and has an officinal history of more than 2000 years in China.FCC has many pharmacological functions such as detoxification,anti-inflammatory,antivirus,lowing hyperlipidemia,antitumour,etc.The main medicinal part is the underground tuberous root.The radicel growing in the FCC is called FCC fibre. The process of FCC is somewhat alike to that of ginseng,in which fibres are removed.The fibres of ginseng are usually made for medicines;however,FCC fibres in the past were mostly abandoned.Reports on Coptis chinensis Franch.about planting,component analysis,pharmacology and so on are exhaustive.But the study of its fibre is still rare.Primary analysis proves that fibres have similar functional components to the root,which suggests that FCC fibre has the same pharmacological function as FCC.If we can exploit and utilize these resources,it will bring about great economic and social benefit.This dissertation mensurates the content of alkaloids in FCC fibre,studied from FCC fibre,according to "Veterinary Pharmacopoeia of PRC.""Pharmacopoeia of PRC.""Dictionary of Chinese herb medicine." and a great deal of interrelated references on general inflammation. Fourteen kinds of Chinese herbs usually used to treat general inflammation were chosen to be compouded with FCC fibre,which are Fruit Cnidium,Radix Sophorae Flavesentis,Cortex phellodendri,Cotex Dictamni,Pulsatilla chinensis(Bunge) Regel,Rheum,Cortex Fraxini,Gentiana scabra,AndrograPhisp aniculata(Burm.f.) Nees,Alumen,Glabrous Greenbrier Rhizome,Flos Caryophyllata,Belvedere Fruit,Radix Scuteuariase.Through in vitro antibacterial test on bacillus subtilis,bacillaceae,tetrads,staphylococcus aureus,E.coli,proteus,salmonella enteritidis, Escherichia coli,bacterial gill-rot disease of fish and fish enteritis bacteria,we examined the antibacterial activity of FCC,FCC fibre and compositions of FCC fibre with each Chinese traditional medicine.Eight Chinese herbs were chosen to be composite with FCC fibre.Test their antibacterial activities.The specific test results are as follows:(1) The content of alkaloids in FCC fibre is2.54%,jatrorrhizine 0.376%,coptisine 1.165%, palmatine 0.071%,and berberine 0.935%;(2)Extracts of FCC,FCC fibre have certain inhibition effects on B.megaterium,B.subtilis, E.coli,P.vulgaris,M.tetragenus,S.aureus,S.enteritidis,Flexibacter calum-naris,Aeromonas hydrophila.and have no inhibition effect on C.tropicalis;The compositions of FCC fibre with each Chinese traditional medicine show inhibition effects on bacteria,some better,some worse.(3)Compound 2 has the strongest antibacterrial activity.The Proportion of compound 2 is A:B:C:D:E:F:G:H:I:J=3:2:2:1:8:1:2:2:3.