| Objective: Acute blunt trauma of the gastronomies’ muscle often occur in sports, such as basketball, volleyball, football and so on competitive strong in sports, exercise capacity of skeletal muscle after blunt trauma will be severely damaged, and blunt trauma repair process is slow, serious and even affect the future sports ability. Homemade blunt injury blow of this study was to use the blunt trauma of rat gastronomies’ mold processing, and anatomy of the gastronomies’ muscle of rats after blunt trauma, observation and measurement to determine the mitochondrial respiratory chain enzyme complexes in rats Ⅰ, Ⅱ, Ⅲ activity changes, for the research of gastronomies after blunt injury recovery and so on to provide more theoretical basis and experimental basis.Methods: 10 weeks Wister male rats, were randomly divided into control group, 12 h after blunt trauma group, 2 group d, 5 d, 7 d, 10 d group, 15 d, 30 d group 8 group(n = 6).In addition to the control group and the standby group are with homemade blunt injury blow will be blunt trauma rat gastronomies’ processing, thus establish a blunt trauma research model.12 h after blunt trauma, 2 d, 5 d, 7 d, 10 d, 15 d, 30 d will kill rats beheaded, respectively take blunt trauma gastronomies to- 80 ℃ freezer for standby. Experimental operation experiment center in Shandong institute of physical education and life science college of Shandong university.Determination of protein enzyme complex and active kit built biotechnology companies are bought from Nanjing. The experimental results are made of SPSS20.0 for repeated measurement variance analysis.Results: 1.mitochondrial enzyme complex I activity in 12 h- 7 d down significantly blunt trauma, to 7 to 15 d activity increased, and the control group with significant difference(P < 0.01);Comparison of the control group in the group with 10 d has significant difference(P < 0.05), 12 h and 5 d, 10 d appeared significant difference(P < 0.05), 2 d and 5 d, 10 d appeared significant difference(P < 0.05), a significant difference(P < 0.05), 12 h, 10 d, 7 d and 15 d appeared significant difference(P < 0.05), 10 d with all the group appeared significant difference(P < 0.05), 15 d and 5 d, 7d, 10 d, 30 d appeared significant difference(P < 0.05), and 12 h, 10 d, 30 d 15 d appeared significant difference(P < 0.05). 2. mitochondrial enzyme complex II activity within 12 h- 2 d time after injury significantly higher(P < 0.05), and then between 5-15 d activity significantly decline compared with control group(P < 0.05).Group in the control group and 12 in the comparison of the h, 2 d, 10 d, 15 d, 30 d has significant difference(P < 0.05), 12 h, 2 d appeared significant difference with control group(P < 0.05), 2 d with all groups have significant, only 7 d and 2 d appeared significant difference(P < 0.05), 10 d, 2 d appeared significant difference with control group(P < 0.05), 15 d, 2 d appeared significant difference with control group(P < 0.05), 30 d, 2 d appeared significant difference with control group(P < 0.05). 3. a mitochondrial enzyme complex III activity within 2-15 d after injury in different degrees of rise, in 2 d and 15 d activity appeared two peak, respectively.Within the group in the control group in the comparison of the 15 d and 2 d, with significant difference(P < 0.05), 12 h and 2 d, 15 d appeared significant difference(P < 0.05), 2 d with the control group, 12 h, 5 d, 7 d, 15 d appeared significant difference(P < 0.05), 5 d and 2 d, 15 d appeared significant difference(P < 0.05), 7 d and 2 d, 15 d appeared significant difference(P < 0.05), 10 d with only 15 d appeared significant difference(P < 0.05), 15 d with all groups have significant difference(P < 0.05), 30 d with only 15 d has significant difference(P < 0.05).Conclusion:1, the activity of mitochondrial respiratory chain enzyme complexes is one of the commonly used biochemical indicators reflect the function of the skeletal muscle, the experimental results showed that the recovery period of 30 days is enough for the damage degree of the experiment, damage can fully recover, the experimental group rats in all kinds of enzyme activity and the activity of normal group rats compared with no significant differences. 2, this experiment proved that produced by blunt injury blow device can be used to production of blunt trauma model. For Wister rats, the average damage degree is more appropriate, so as not to cause the rats’ adverse situation such as severe injuries, fractures, death, compared with the research purpose of this experiment. 3, blunt trauma can induce mitochondrial enzyme activities of compounds, but different complex enzyme activity change time is different. 4, complex enzyme activity changes reveal respectively in mitochondria damage after 12 h- 2 d and7-15 d provide effective energy supply, may be the key stage of recovery after blunt trauma. 5, according to different stages of enzyme activity change in after blunt trauma in the repair process, there may be a primary electron transport chain vicarious conversions between hydrogen donor, embodies the mitochondria of energy supply of adaptive changes after blunt trauma. |
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