| Purpose: This study through oxidative phosphorylation theory and on the basis of experiment animal obesity rat model of hypoxia, explores the effect of low oxygen environment and training on body weight of obese rats, studies the relationship between respiratory oxygen consumption and energy synthesis in the brain tissue of the brain during hypoxic exercise, elucidates the association between respiratory oxygen consumption and energy supply in brain tissue during hypoxic exercise, understands the principles, clears the law of its transformation, provides theoretical basis for the practical application of the low oxygen movement, provides scientific guidance to the people involved in the movement to lose weight, and provides theoretical basis for the scientific training of aerobic endurance training methods.Methods: Chose 120 SD male rats whose age is six weeks to feed in different cages according to the experimental requirement.(1) Environmental adaptation: In order to make the rats be familiar with the feeding environment, feed the rats quietly for a week when they are put into the lab first;(2) Swimming adaptation: The rats in each group finish six-day swimming adaptive training under the condition of constant oxygen, which the time is for ten minutes;(3) Exercise intensity adaptive training lets the rats adapt the swimming training intensity, and training time begins at fifteen minutes which adds five minutes everyday, and at last the speed reaches at thirty-five minutes, a total of six days;(4) Hypoxia adaptive training: At first the rats are exposed to hypoxia to have adaptive training for 2 days, and the swimming time continues for 15 minutes; then increase the adaptive strength, and the training time increases from 15 minutes to 30 minutes, and the adaptive training continues for 4 days. After the animals having adaptive feeding in feeding room for 2 weeks, they were divided into six groups for Group A, Group B, Group C, Group D, Group E and Group F randomly, which Group A is ordinary movement(n=20); Group B is common control(n=20); Group C is high fat exercise(n=20); Group D is high fat control(n=20); Group E is high fat and low oxygen movement(n=20); Group F is high fat and low oxygen control(n=20). The hypoxia group was placed in the hypoxia chamber to simulate the altitude of 3400 m altitude(atmospheric pressure 61.6 k Pa, temperature 25 degrees Celsius) for 8 weeks. Control group of animals are at an altitude of 400 meters of the plain(atmospheric pressure 96.6 k Pa, room temperature 25 degrees Celsius), without for hypoxia stimulation.(5)The standard of selecting the animals: Through the body weight, swimming adaptation and the hypoxia adaptation eliminated the rats which couldn’t meet the experimental requirements. At last leaves 82 rats to complete the formal experiment. Group A is ordinary movement(n=11); Group B is common control(n=11); Group C is high fat exercise(n=15); Group D is high fat control(n=15); Group E is high fat and low oxygen movement(n=15); Group F is high fat and low oxygen control(n=15).Results:(1) There were no significant differences in body weight of the rats in normal group. There was no significant difference in body weight of rats in high-fat group, and there was a significant difference between the body weight and the body weight of the rats in general group and obesity group; After training, the body weight of the rats in the normal group and the high fat group was significantly increased compared with that before the training. Compared with the normal group(A and B), there was no significant change before and after the training, but the weight of the exercise group was lower than that of the control group. Compared with the high fat group(C, D, E, F) the increase in training before and after are no significant differences. However after training(Group C and Group D, Group E and Group F) were lower than those of control rats gained weight, and high fat in hypoxia group and high fat often oxygen group compared, and hypoxic exercise and control group of obese rats body weight were lower than regular aerobic exercise and control group of obese rats weight.(2)Through analyzing the experimental data results of ATP, ADP, AMP of the rats’ cerebral mitochondria we can know that: Group A’s AMP content is higher than Group C, and there were significant differences(P<0.05); Group E’s ATP content is higher than Group A, and there were significant differences(P<0.05); Group E’s ATP, ADP, AMP content are higher than Group C, and there were significant differences(P<0.05). Group D’s ATP and ADP content is higher than Group F, and there were significant differences(P<0.05). Group C’s ADP is higher than Group D through exercise intervention and there were significant differences(P<0.05). Group E’s ATP is higher than Group F through exercise intervention and there were significant differences(P<0.05).(3)Through the mitochondrial ATP enzyme activity test we can know that: the Group C’s enzymatic activity of Mg-ATP、Ca-ATP、Ca,Mg is lower than Group D through exercise intervention and there were significant differences(P<0.05).(4)Through the mitochondrial oxidative respiratory activity test we can know that: the Group E’s RCR、OPR content is lower than Group A, and there were significant differences(P<0.05). Group D’s RCR content is lower than Group B, and there were significant differences(P<0.05). Group C’s ST3(the three state of the mitochondria in the brain of rats after acute hypoxia 24h) is higher than Group D through exercise intervention and there were significant differences(P<0.05). Group E’s ST4(four state of respiration) is higher than Group F through exercise intervention and there were significant differences( P<0.05).(5)Through the mitochondrial membrane potential test we can know that: exercise intervention increased mitochondrial function and energy metabolism was more active, but there was no significant difference between the groups.Conclusions:(1) Exercise can inhibit the increase of body weight in obese rats, and the effect of hypoxia training(oxygen concentration14%) on weight gain will the same effect.(2) Hypoxia training(oxygen concentration14%)makes brain mitochondrial oxidative phosphorylation efficiency become higher, increases the ATP synthesis, and stimulates the organism to compose the energy materials.(3) Exercise increases the oxygen transport capacity of the body, while the hypoxic training(oxygen concentration14%) has an advantage, and the effect is the best.(4) Exercise can improve the efficiency of mitochondrial respiratory chain in brain tissue, while the stimulation of hypoxia(oxygen concentration14%), but exercise can increase the efficiency of mitochondrial oxidative respiratory chain in brain tissue. |
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