Chemical Components And Bioactivities Of Amoora Tetrapetala(Pierre) Pellegr. And Swietenia Macrophylla King

Amoora tetrapetala belongs to the genus of Amoora (Meliaceae) and Swietenia macrophylla belongs to the genus of Swietenia (Meliaceae). Researches have shown that these two species are rich in limonoids compounds. In order to search for more biologically active compounds, the studies of the Amoora tetrapetala and Swietenia macrophylla, which both were from Hainan, were performed by all kinds of separation methods and spectral analysis. Furthermore, the biological activities of the compounds were tested, which may provide a scientific basis of the development and utilization of the botanical resources.Fourteen compounds were isolated from the branches of Amoora tetrapetala and structurally identified by spectral analysis. The compounds were identified as22?-hydroxytirucalla-7,24-dien-3,23-dione (1), dymacrin D (2), zeorin (3), ?-spinasterol (4),8-hydroxy-6-methoxy-3-pentylisocoumarin (5), lichenxanthone (6),10-oxo-isodauc-3-en-15-al (7),(+)-ent-ficusol (8),3-methoxy-4-hydroxybenzoate (9), vanillin (10), methyl2,4-hydroxy-3,6-dimethylbenzoate (11), methyl2-hydroxy-4-methoxy-6-propylbenzoate (12), methyl2,4-dihydroxy-6-methylbenzoate (13) and methyl hydrogen succinate (14). All of the compounds were isolated from this genus for the first time.Twenty-four compounds were isolated from the roots of Swietenia macrophylla and structurally identified by spectral analysis. The compounds were identified as (+) catechin (15),1,5-dihydroxyxanthone (16),1,6-dihydroxy-5-methoxyxanthone (17), euxanthone (18),1,2-dimethoxyxanthone (19),6-deoxyjacareubin (20),1,5-dihydroxy-6-methoxy-6'6'-dimethyl-2H-pyrano[2',3':3,2]xanthone (21),(+)sesamin (22),3-oxotirucalla-7,24-dien-21-oic acid (23),(20S)-3?-acetoxy-24-methylenedam-maran-20-ol (24), cycloeucalenol (25), a-hexy-3-(6-hydroxy-2,4-ocadiynyl)oxiranemethanol (26), hexane-9-ene-4,6-diyne-3,11-diol (27),6,7,8-trihydroxytetradeca-1,3-diyne (28),10-methoxyheptadeca-4,6-diyne-3,9-diol (29), heptadecyl-8-ene-4,6-diyne-3,10,11-tirol (30),(3R,8E,10S)-heptadec-8-ene-4-diyne-3-diol (31), swieterootphragmin A (32),12a-acetoxyswietephragmin C (33), swietephragmin C (34),7-deacetoxy-7-oxogedunin (35),7-deacetoxy-7?-hydroxygedunin (36),bis-(2-ethylhexyl) phthalate (37) and ?-sitosterol (38). Among them, six new compounds, including one xanthone (21) and four acetylenic compounds (27,28,29and30), along with one limonoid (32).The antibacterial activities of compounds1-38were measured by the method of using paper disco diffusion. The result indicated that compound14had a certain inhibitory activity against Staphylococcu aureus. Compounds11and13showed some inhibitory activities against Canidia albicans and Fusarim noxysporun f. sp. cuben. Compound14had activity against Ralstonia solanaceanum. Compounds4and11had activities against Fusarim noxysporun f. sp. vasinfectum.Using MTT method to evaluated cytotoxicities of compounds and the result showed that26and27have cytotoxicities against SGC-7901and BEL-7402as well as compound28to SGC-7901and K562.The acetylcholinesterase inhibitory activities of compounds1-38were tested according the method of Ellman, and through the result we can see11,20,26and27have ACh E inhibition and were supposed to pose the function of neuroprotective.