Chinese Medicine Decoction On The Analysis Of The Metal Ion Binding Ability Evaluation Research

The content is lower than 0.01% of body weight referred to trace elements. Although its content is low, it plays an important role in the body to maintain physiological functions. When the trace element is lack or excess, the body will be lesioned. For example, Wilson's disease and mercury poisoning associated with trace copper and mercury excess. It is difficult to control the income of excess trace elements because the air, water and some times food pollution. If some ligands can be found from herbs or foods, they can chelate with excess trace elenment and excrete from body. The harmful effects of excess trace elenments will be reduced. An analytical method was needed to screen and evaluate the chelating ability of the trational Chinese medicine.The mercury ion can be chelated with the diphenylcarbazide ?DPC? and extracted with methylene dichloride-ethanol, then determined by spectrophotometor at 554 nm.The copper ion can be chelated with 1-?2-pyridylazo?-2-naphthol ?PAN? and extracted with methylene dichloride-ethanol, then determined by spectrophotometor at 600nm.The vanadium ion can be chelated with tantalum reagent ?BPA? and extracted with methylene dichloride-ethanol, then determined by spectrophotometor at 445 nm. Hg²⁺?1.4-14.0 mg·L-1? were determined with good accuracy and reproducibility; the detection limit was 0.0070mg·L-1. Cu²⁺?0.64-6.40 mg·L-1? were determined with good accuracy and reproducibility; the detection limit was about 0.046 mg·L-1. VO₃^-?0.21-10.18 mg·L-1? were determined with good accuracy and reproducibility; the detection limit was about 0.0018 mg·L-1.The contents of metal ions in decoction of traditional Chinese medicine were determined by the method established above. Then certain amounts of mercury ion, copper ion and vanadium ion were added to the decoctions and the recovery of the added ions was determined respectively. The recovery was reverse correlation with chelating ability of decoction of traditional Chinese medicine.The chelating ability of traditional medicine with mercury ion, copper ion and vanadium ion was evaluated. The decoctions of Rheum palmatum L., Millettia reticuiata Benth., Rosa rugosa Thunb., Platycodon grandiflorum ?Jaoq.? A.DC., Angelica sinensis ?Oliv.? Diels, Lysimachia christinae Hance, Houttuynia cordata Thunb., Paeonia lactiflora Pall., Cinnamomum cassia Presl., Smilax glabra Roxb., Psoralea corylifolia L., Carthamus tinctorius L. posess strong chelating ability with mercury ion ?80.26%-91.14%?. The decoction of Rosa rugosa Thunb., Lysimachia christinae Hance, Smilax glabra Roxb., Artemisia capillaris Thunb., Scutellaria baicalensis Georgi., Cimicifuga foetida L., and Houttuynia cordata Thunb. posess strong chelating ability with copper ion ?56.19%-84.63%?. The decoctions of Rosa rugosa Thunb., Artemisia capillaris Thunb., Gardenia jasminoides Ellis., Houttuynia cordata Thunb.,Sa/v/a miltiorrhiza Bge., Trichosanthes kirilowii Maxim., Paeonia suffruticosa Andr.posess strong chelating ability with vanadium ion ?51.31%-92.44%?. The developed method can be used to evaluate the chelating ability of decoctions of traditional Chinese medicine with mercury ion, copper ion and vanadium ion.