Study On Vancomycin And Teicoplanin Used As Chiral Separation Materials

The chirality,the methods of chiral recognition,the significance of chiral separation,the membrane preparation,the membrane separation and the per aqueous liquid chromatography were briefly introduced.The vancomycin-benzenetricarbonyl trichloride polyethersulfone composite membranes were prepared by interfacial polymerization reaction.Some parameters of membrane preparation were optimized.When the aqueous solution concentration of vancomycin was 1.4g/L and the immersion time was 26 h,the n-heptane solution concentration of 1,3,5-benzenetricarbonyl trichloride was 11g/L and the immersion time was 25 h,the dialysis time was 3 days,the temperature of heat treatment was 80℃,the optical resolution membranes reached 57.3 %e.e.for 0.4mg/m L D,L-phenylglycine,35.9 %e.e.for 0.2mg/m L D,L-p-hydroxyphenylglycine,60 %e.e.with large size membrane for D,L-phenylglycine,respectively.Under the optimization conditions of preparation membrane,the teicoplanin-benzenetricarbonyl trichloride polyethersulfone composite membranes were also prepared.37.5 %e.e.for 0.2mg/m L D,L-p-henylglycine,37.8 % e.e.for 0.3mg/m L D,L-p-hydroxyphenylglycine,40 %e.e.with large size membrane for D,L-p-henylglycine were also obtained.We studied the chiral membrane chromatography on vancomycin-benzenetricarbonyl trichloride and teicoplanin-benzenetricarbonyl trichloride polyethersulfone composite membranes with mobile phases of water,different proportions of methanol/water and pure methanol.By the per water liquid chromatography experimental conditions,the enantioseparations were carried out by commercial columns and home-made columns of vancomycin and teicoplanin.Some amino acids such as D,L-phenylglycine,D,L-p-hydroxyphenyl glycine and D,L-methionine were separated.