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Fluorescence Spectrum And Transmission Spectrum Method For Blood Samples Components Measurement

Posted on:2018-03-18Degree:MasterType:Thesis
Country:ChinaCandidate:Z Y LiFull Text:PDF
GTID:2321330542981383Subject:Biomedical engineering
Abstract/Summary:PDF Full Text Request
As is well known to all,the variation of hematological biochemical parameters plays a crucial role in the clinical diagnosis and treatment.Many diseases may lead to variation of the blood parameters or characteristic properties.In recent years,noninvasive measurement blood component has become a hotspot in the field of biomedical engineering.Spectroscopy,because of its characteristics of convenience,noninvasiveness,multi-dimensional information,high speed and high accuracy,has become the most promising method both for in vitro or in vivo.Based on the elaboration of the principle of fluorescence spectrum and dynamic spectrum,this dissertation mainly include following parts.Firstly,we designed an experimental facility for fluorescence spectrum measurement with a 365nm UV LED,and the whole blood and plasma with different fHb concentrations were provided by the Institute of Transfusion Medicine of the Academy of Military Medical Sciences.We measured the transmission spectrum of whole blood and the transmission spectrum and fluorescence spectrum of plasma.And the correlation coefficients(R_p)of whole blood transmission spectra,plasma fluorescence spectra,and plasma transmission spectra were up to 0.9524,0.9774,and0.9729,respectively.The high-concentration of whole blood can result in the serious reabsorption of fluorescence spectra,leading to a weak signal.However,the three existing measuring results have been validated in clinical fHb concentration detection.Use of transmission spectra or fluorescence spectra with the PLS method was demonstrated to be a powerful tool for fHb concentration measurement with a high degree of accuracy.Secondly,we designed a measuring equipment for DFS measurement with365nm UV LED.In vivo measurements were carried out on 10 healthy volunteers,and their intrinsic spectrums were collected.Then we extracted valid DFS with single-trial estimation in time domain method.Moreover,a 365nm UV-LED was used as the exciting source to measure the fluorescence emission spectra(FES)of human whole blood and serum.After comparing DFS and FES of serum,we found they have similar fluorescence bands approximately.It confirmed that DFS did reflect the blood component concentration which has fluorescence characteristics.Theoretical analysis and experiment results verified the theoretical possibility of DFS method for noninvasive measurement of blood component concentrations preliminarily.In addition,a co-modeling method based on DS and DFS will enhance the prediction accuracy and enlarge the scope of blood components noninvasively in future studies,and play the role of complementary advantages.Based on the feasibility of fluorescence spectrum and DFS,this dissertation presents a series of measures and methods including signal detection,spectral processing and modeling,to improve the accuracy and sensitivity of spectra compared with the traditional method.All of these will provide ideas and reference directions for other related field.
Keywords/Search Tags:Free hemoglobin, Fluorescence spectrum, Partial least squares method, Noninvasive measurement of blood components, Dynamic fluorescence spectrum
PDF Full Text Request
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