| Litsea coreana var.lanuginosa belongs to Litsea's genus of Lauraceae's familyplant, is a evergreen small tree plants, and it is the raw material plant ofcharacteristic tea drink Laoying Tea which has high nutritional value and great marketprospect in Guizhou province. At present the bottleneck problem of large-scalecultivation Laoying tea plants are scarcing resources of raw material plants andlacking of efficient seedling propagation techniques. The effective seedlingpropagation method of L. coreana is cutting seedling, but now the cutting survivalrate is low. In order to provide theoretical reference for establishing the efficientcutting seedlings technology system of Litsea coreana var.lanuginosa, the systematicstudies on rooting mechanism of cutting propagation of the L. coreana var.lanuginosawas conducted in this paper. The main results were as follows:1.The annual germination regularity of L. coreana var.lanugin-osa shootsThe shoots of L. coreana var.lanuginosa germinated twice in a year.Springshoots buds at the end of March and states the half lignification in the middle of June,at the end of July and early August states completely lignification;autumn shootsbegan to sprout in the middle and late August,in September,it states halflignification,after late October it entered into the state of lignification. Branches ofspring shoots growth than autumn's.2. The embedded substance change rule of L. coreana var.lanug-inosa new shootsSoluble sugar content of L. coreana var.lanuginosa branches in new shoots israther changeable in each individual month, soluble sugar content of New shoots inthe state of half lignification is high than lignification and not lignification,Springshoots's content overtops autumn shoots; protein content of L. coreana var.lanuginosabranches in new shoots is not changing much every month,Spring shoots's proteincontent is lower than those of autumn shoots; the branches of L. coreanavar.lanuginosa total phenolics content has great load changes every month, Newshoots total phenol content is lowest in June to July.Mature leaf chlorophyll content of L. coreana var.lanuginosa of each month hascertain change, lignification of shoot leaf chlorophyll content is high than halflignification and not lignification.The peroxidase (POD) activity of L. coreanavar.lanuginosa has big change in different growth phase,From May to October fornew shoots the peroxidase (POD) activity is reduced,in October to December,lignification degree of new shoots increased, POD activity increased from January toMarch;the extent of variation of spring shoots with the growth stage Catalase(CAT)activity has great chang than autumn shoots, spring shoots CAT activity is high than autumn shoots, CAT activity of spring shoots and autumn shoots were minimumin no lignification stage, half lignification phase center, lignification stage has highestCAT activity, autumn shoots' CAT activity decreased in cold season;PolyphenolOxidase (PPO) activity of spring shoots is higher than autumn shoots, spring shootsand autumn shoots has different change trend, as new shoots go into half lignificationand totally lignification, autumn shoots PPO activity change in the general trend isgradually rised, with new shoots enter into half lignification and lignification,autumnshoots PPO activity is higher and more stable in the low temperatureseason.Superoxide dismutase (SOD) activity of L. coreana var.lanuginosa springshoots was lower than those of autumn shoots, SOD activity is stable in the springgrowth stage,then growth stage in the autumn of SOD activity change is bigger, SODactivity of autumn shoots increased quickly after the lignification,autumn shoots SODactivity is higher and more stable in cold season; malondialdehyde (MDA) content ofLitsea coreana var.lanuginosa spring shoots was lower than those of autumn shoots,malondialdehyde content appear to be gradually rising during spring shoots began tosprout with the process of autumn enter to lignification state, the growth period ofautumn shoots,malondialdehyde content change in a spring shoots is fast, autumnshoots malondialdehyde content is higher and more stable in cold season. The contentof proline was affected by branches state and temperature of L. coreanavar.lanuginosa new shoots, spring shoots and autumn shoots are showed that prolinecontent of not lignification branches is higher than half lignification and lignificationbranches, proline content in autumn shoots is higher than the spring pin, prolinecontent of autumn shoots great increased in cold season.Spring shoots and autumn shoots of L. coreana var.lanuginosa are showed thatendogenous IAA content of half lignification branches is higher than not lignificationand lignification branches, endogenous IAA content in autumn shoots is higher thanthe spring pin,autumn shoots has lower endogenous IAA content in coldseason.Change trend of Endogenous ABA content is different between spring shootsand autumn shoots of L. coreana var.lanuginosa, the spring shoots showedendogenous ABA content of not lignification is higher than half lignification state andlignification, autumn shoots showed it grows gradually rising trend with neverlignification branches to half lignification and lignification, endogenous ABA ofcontent autumn shoots is higher, but not stable in cold season.The growth anddevelopment rule of L. coreana var.lanuginosa New shoots is closely related to itsendogenous gibberellic acid (GA) levels, endogenous GA content is the lowest in halflignificationl state of new shoots.endogenous GA content of spring shoots halflignification stems is higher than that of autumn shoots, endogenous GA content werehigher,during stirring in the new shoots with germination, elongation growth andlignification, with the temperature decrease, autumn shoots grow slowly andendogenous GA content also gradually decline. Endogenous cytokinins ZR content of new shoots of L. coreana var.lanuginosa sharply volatile; endogenous ZR content ofnot lignification stems in half a high state oflignification and lignification; Autumnshoots content is higher than spring shoots; The cold season, autumn shootsendogenous ZR content showed a downward trend.3. The influence of mother tree age on cuttings rooting ability and theembedded substance content of L. coreana var.lanuginosaMother tree age has significantly influence on the cuttings rooting ability ofcutting shalf lignification of L. coreana var.lanuginosa, adult mother tree cuttingsrooting rate of cuttings is highest,72.45%,significantly higher than that of young andmiddle-aged trees's rooting rate of cuttings.Insignificant difference of Young andmiddle-aged trees cuttings rooting rate of cuttings,Rooting rate were63.10%and65.14%respectively.Mother tree age has no effect on the content of soluble sugar and protein of halflignification cuttings of L. coreana var.lanuginosa, but significant impact on totalphenol content;there are significant difference on adult mother tree and middle-agedtrees cuttings of total phenol content, The cuttings of young mother tree and adulttrees and middle-aged trees there was no significant difference of total phenol content.Mother tree age has no significant influence on photosynthetic capacity of halflignification of L. coreana var.lanuginosa cuttings, different mother tree age has nosignificant difference on chlorophyll content of half lignification cuttings leaf.Mothertree age significantly affect on peroxidase (POD) and catalase (CAT) activity of halflignification cuttings of L. coreana var.lanuginosa,then has no effect on the activity ofpolyphenol oxidase (PPO).Mother tree age significantly affect on half lignificationcuttings of L. coreana var.lanuginosa with the content of malondialdehyde andproline, these content of adult mother trees cuttings is lowest.Mother tree age has no significantly effect on endogenous auxin IAA, abscisicacid, ABA and cytokinin ZR content of half lignification cuttings of L. coreanavar.lanuginosa,but it has significant difference of the content of endogenous GA.4. The influence of matrixes on cuttings rooting ability and theembedded substance content of L. coreana var.lanuginosaMatrix significantly affec on rooting ability of half lignification cuttings of L.coreana var.lanuginosa, matrix of native purple shale silty loam of Laoying tea,cuttings rooting rate of cuttings on top,is70.64%.Matrix has no effect on the content of soluble sugar and protein of halflignification cuttings of L. coreana var.lanuginosa, but significant impact on totalphenol contentMatrix has no significant influence on photosynthetic capacity of halflignification cuttings of L. coreana var.lanuginosa, different matrix has no significantdifference on chlorophyll content of the leaf.Matrix has no effect on the activity of peroxidase (POD),but significantly affect on polyphenol oxidase (PPO)and catalase(CAT) activity and the antisenility physiological indexes of SOD of half lignificationcuttings of L. coreana var.lanuginosa.CAT activity of the pearlite matrix cuttings ishighest,PPO activity of vermiculite matrix cuttings is highest, vermiculite matrixcuttings on the highest SOD activity;The proline and malondialdehyde content ofdifferent matrix cuttings is significant difference,the malondialdehyde content ofpearlite matrix on cuttings is lowest, proline content of cuttings is the lowest on thePurple sand substrate.Matrix has significantly effect on endogenous auxin IAA, abscisic acid, ABAand cytokinin ZR content of half lignification cuttings of L. coreana var.lanuginosa,but there are no significant difference on the content of endogenous GA.Theendogenous IAA and ZR contents are the lowest of cuttings on purple sand matrix,the cuttings of endogenous ABA content is the lowest on the purple sand substrate.5. Dynamic change rule of embedded substance in the process ofcuttings rooting of L. coreana var.lanuginosaMorphological chang rule of Cuttings under snip basically the same in theprocess of cuttings rooting, after processed by clear water and growth regulators IBA,GGR and GA of half lignification cuttings of L. coreana var.lanuginosa. after cuttingin10~20days, it is the callus differentiation stage of cuttings; after cutting in20~60days,that is period of creating for the callus; after cutting in60~80days, it isdifferentiation phase of roots;after cutting in80~100days, which is the phase ofcreating roots system. Hormone type and concentration significantly impact oncuttings rooting of L. coreana var.lanuginosa, differentiation of callus and roots ontreatment effect and time appeared differences after processed by different hormone;cuttings rooting rate of half lignification cuttings of Litsea coreana var.lanuginosa,hormone treatment of15mg·L-1IBA is the highest, rooting rate is94.44%. Most of thehormone treatment rooting rate under70%, controlled clear water treatment ofcuttings rooting rate is only30.00%.Soluble sugar content in all processing cuttings showed reduction trend in the10~40days,after cutting of half lignification cuttings, cuttings for40days, the processof callus producing and root differentiation, soluble sugar content of contrast andmost volatile hormone treatment cuttings in a significant fluctuation,only the besthormone treatment IBA15cuttings of soluble sugar content is relatively stable.Afterall hormone treatment of cuttings for40days,protein content of cuttings dropped tothe lowest, and then gradually rised, slowly declined again in60days, which hassmall change;contrast in cutting of10days later, the protein content of cuttings hasplummeted to the lowest, reached the highest in40days, after40days slowed down,change is smaller.All hormone treatment and compared of cuttings in40days aftercutting, total phenol content of cuttings down to the lowest, to60days, and a sharprise to the peak levels, then slow down. Hormone treatment of half lignification cuttings after cutting, chlorophyllcontent of cuttings leaf present the downward trend, most of chlorophyll content ofthe hormone treatment in the process of cutting rooting of cuttings volatile, onlychlorophyll content of cuttings of the best hormone treatment IBA15and contrast incuttings, declined slightly after20~40days, then held steady after the late.Afterhormone treatment cutting, POD activity of cuttings present the rising trend,cuttingsgenerated callus and root, POD activity of cuttings increased significantly, the peakperiod of POD activity and callus produce period and root producing phase of theprocessing cuttings is synchronizated, half lignification cuttings of L. coreanavar.lanuginosa after processing with different hormones, the change of CAT activityof the cuttings is bigger in cuttings after10~20days, CAT activity of cuttings ofcontrast and most of the hormone treatment increased significantly,only CAT activityof growth regulators IBA treatment cuttings decreased obviously, the best hormonetreatment IBA15dropped to a low in10days after cutting, and most of the processingon the CAT activity after cutting to fall to a relatively low in40days; halflignification cuttings of L. coreana var.lanuginosa of different hormone treatmentafter cutting during10~20days, PPO activity of contrast and handle the cuttingsbegan to rise, the best hormone treatment IBA15cuttings PPO activity is higherthan other treatment and contrast energy,during cutting40~60days, the cuttingcortex began to produce callus, CAT activity of hormone treatment and the contrastwas down to low,60~80days after cutting, the period for differentiation root systemof callus, PPO activity of control and almost hormone treatment cuttings began to rise,PPO activity of the best hormone treatment cuttings is the lowest.All processingcuttings of SOD activity present overall rising in the process of cutting rooting,0~40days, most of the processing of cutting SOD activity is stable, SOD activity of almostprocessing of cuttings increases in40days, the best hormone treatment IBA15andmost treatment peaked in80days,Only a few processing such as IBA500reached itspeak within60days of cutting SOD activity, control the cutting SOD activity, thereare two peak, respectively in callus produce period and root generating period.Allprocessing the malondialdehyde content of cuttings and SOD activity has consistenttrends in the process of cutting rooting, the overall rise;processing and comparisonclose to content of malondialdehyde, less volatile ups and downs.Proline content ofhalf lignification cuttings of L. coreana var.lanuginosa after cutting,which is on therise in general, proline content of cuttings of contrast and most processing is on therise in20~40days after the cutting,Only the best hormone treatment IBA15,proline content of cuttings to reach the first peak within20days after cutting,period ofcallus producing in40~60days, only the best hormone treatment IBA15cuttings ofproline content down to a lower level,Period of producing root in80~100days, atthis point the best hormone treatment IBA15cuttings of proline content is lower thanmost of hormone treatment and control cuttings. Fluctuation of endogenous auxin IAA content of hormone treatment cuttings,endogenous IAA content of different treatment of cuttings has different change trend,the change rule is not obvious.By analyzing change rule of the endogenous IAAcontent of cuttings between the best hormone treatment IBA15and contrast, foundthe endogenous IAA content of half lignification cuttings of L. coreanavar.lanuginosa is higher by itself, is not conducive to the differentiation of callus androot of cuttings;Best hormone treatment IBA15cuttings of endogenous IAA contentdropped significantly in period of differentiation of callus and root cutting,significantly improved the the rate of callus and root of cuttings.Endogenous abscisicacid ABA content of contrast and hormone treatment cuttings is ratherchangeable;growth regulators IBA and GGR effect on the endogenous ABA contentof cuttings is different,after cutting in10days, the majority of differentconcentration of IBA treatment cuttings of endogenous ABA content increasedsignificantly,And most of the different concentrations of cuttings in endogenous ABAcontent of aboveground clumps treated with endogenous GGR significantlyreduced;All processing are showed characteristics in the period of callus and rootproducing, has low content of endogenous ABA of cuttings.Cuttings of L. coreanavar.lanuginosa with exogenous hormones processing after cutting, in addition toendogenous GA content of IBA25treatment and controlled cuttings is slightly lower,the improvement of the rest of the process to some extent;Cuttings after20days, inaddition to endogenous GA content of IBA250processing cuttings is higher than thereference cuttings, the rest of the endogenous GA content of cuttings are lower thancontrol cuttings, endogenous GA content of IBA25and IBA15processing cuttings islow.In combination with the cuttings rooting situation, found that more than50%ofprocessing cuttings rooting rate in80days after cutting, endogenous GA content ofcuttings are low.Endogenous cytokinins ZR content of cuttings of L. coreanavar.lanuginosa greatly change,By analyzing the change trend of endogenous ZRcontent of IBA15and GGR50processing,which has higher rooting rate,and controlcuttings,Found the endogenous ZR content of cuttings is low, which is advantageousto the differentiation of callus and root, after producing the callus and root, thecreation of a high level of endogenous ZR content of cuttings is conducive to growthof cell division of callus and root; adjustment-control in a different way ofendogenous ZR content of cuttings of L. coreana var.lanuginosa treated by growthregulators IBA and GGR, endogenous ZR content of growth regulators IBA treatmentcuttings change trend of waves, growth regulator cuttings of aboveground clumpstreated with GGR endogenous ZR content in a slow gentle upward trend afterplummeted.6.The correlation of embedded substance and the cuttingsrooting ability of cuttingsThe correlation analysis of nutrients content and callus differentiation ability of half lignification cuttings of L. coreana var.lanuginosa, showed that the soluble sugarcontent and protein content of cuttings and differentiation of callus ability of cuttingsat the0.1level negative correlation;it showed that the soluble sugar, protein contentof cuttings and the ability of cuttings root differentiation had no significant correlation;cuttings of total phenol content and the differentiation of callus and differentiationability of root of L. coreana var.lanuginosa cuttings,were significantly negativecorrelation at the0.05level.The leaf of chlorophyll content, malondialdehyde content, proline content andSOD, CAT and POD activity of half lignification cuttings of L. coreanavar.lanuginosa,these six physiological indexes and differentiation of callus ability ofcuttings had no obvious correlation;But the polyphenol oxidase activity significantlypositive correlation with differentiation of callus ability of cuttings at the0.05level.Correlation analysis between physiological indexes and cuttings rootdifferentiation ability,showed that only the SOD vigor and the ability ofdifferentiation of cuttings root significantly negative correlation at the0.05level, therest of the physiological indicators and the differentiation of the root of cuttings hadno obvious correlation.The correlation analysis of endogenous hormone content and callusdifferentiation ability of half lignification cuttings of L. coreana var.lanuginosashowed that the endogenous hormones IAA and GA of cuttings and the differentiationof callus of cuttings ability has no obvious correlation, endogenous hormones ABAand ZR of cuttings and the differentiation of callus of cuttings capacity weresignificantly negative correlation at the0.05level.that in addition to the endogenousZR content of cuttings is negatively related to the ability of cuttings rootdifferentiation at the0.1level, three kinds of endogenous hormone IAA, ABA, andGA has no significant effect on the ability of differentiation of cuttings root. |
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