Preliminary Study On Tissue Culture Of Two Ornamental Bamboos

In this paper, bamboo shoots, new tender branches, new germination buds, dormant buds and rhizome of Phyllostachys pubescent cv. Tubaeformis were tested as explants; and new tender branches, dormant buds of Phyllostachys pubescent f. heterocyclic were tested as explants in order to probe into the influence of different sterilized time on explants. Different basic medias, regulators, accessories and light conditions were studied in the course of inducement, multiplication and germination of buds and the main influence factors and effective concentration scale were analyzed. The bamboo shoots, new buds were investigated in order to find the main influence factors and effective concentration scale in the inducement course of callus. The major results are as follows:1. Phyllostachys pubescent cv. Tubaeformis(1) The best sterilizing effect on new tender branches segments is 15 second with 75% ethanol solution and 6 minutes with 0.1% HgCl2. The most suitable culture medium for bud induction is 3/4MS+KT0.5mg/L +TDZ0.001mg/L(2) The best sterilizing effect on new germination buds is 20 second with 75% ethanol solution and 6 minutes with 0.1%HgCl2. The most suitable culture medium for bud induction is 1/2MS+ KT0.5mg/L+TDZ0.001mg/L. The most suitable lighting conditions for 10 days is before 24h/d dark, and then transferred to 12h/d light+12 h/d of darkness.(3) When adding PVP100mg/L+Vc40mg/L to the culture medium for induced semi-lignified dormant buds, the explants has the lowest browning rate.(4) In terms of cultivating the Phyllostachys pubescent cv. Tubaeformis of different explants induction, the best effect was the new tender branches.(5) The optimizing medium for subculture multiplication is 1/2MS+0.5mg/L KT+TDZ0.001mg/L.(6) By adding PVP100mg/L+Vc40mg/L to the culture medium for induced callus, the explants has the lowest browning rate. 2. Phyllostachys pubescent f. heterocyclic(1)The best sterilizing effect on new tender branches segments is 20second with 75% ethanol solution and 7 minutes with 0.1% HgCl2. The most suitable culture medium for bud induction is 3/4MS+KTO.5mg/L +TDZ0.001mg/L.The most suitable lighting conditions for 10 days is before 24h/d dark, and then transferred to 12h/d light+12 h/d of darkness.(2) The semi-lignified dormant buds in cysteine (100 mg/L) immersion after 2 hours and then with normal disinfection, digging into medium by adding PVP (100mg/L)+Vc (40mg/L), the explants has the lowest browning rate.