Establishment Of Anthirrhinum Majus Regeneration System And Research Of Its Phyllotactic Changing Rules Of Vegetative Propagation Offspring

Anthirrhinum majus is one of the plants which have two or more types of phyllotaxy, and its phyllotaxis will change with the growth. The leaf development mechanism is very complex. The relevant literatures indicate that the exogenous phytohormone play an important role in leaf development and regulation of phyllotaxy patterns. In this study, we tried to establish high efficiency regeneration system of Anthirrhinum majus, and discussed the changing rules of phyllotaxis of vegetative propagation offspring by two ways, one of which is tissue culture and another one is cutting propagation. Results are as follows:1.The shoot tip of Anthirrhinum majus was used as explants to study the effects of different exogenous phytohormone on the establishment of its regeneration system. The results showed that the optimum multiplication medium was MS+1 mg/L 6-BA+0.1 mg/L NAA, which inducing a large number of better growing clumpy buds; And the best quality of clumpy buds’ root was showed in 1/2 MS rooting medium without phytohormon but had a 20 g/L consistence of cane suger.2.In this study, the shoot tip with opposite phyllotaxy and the shoot tip with alternate phyllotaxy were used as explants to produce clumpy buds on culture mediums with different concentration of 6-BA and NAA, we tried to find out the development features of the clumpy buds phyllotaxis by observing. The result showed that when the shoot tip with opposite phyllotaxy were used as explants and the concentration of 6-BA remained unchange, exogenous NAA application could induce a change in phyllotaxy pattern from opposite to alternate; Up to a point, the higher NAA concentrations, the higher proportion of alternate phyllotaxy pattern; The plants with ternate arrangement phyllotaxy pattern were generated on the culture mediums with 1.0 mg/L 6-BA; Without any exogenous phytohormone in rooting medium, the alternate leaf primordium would come back to the original phyllotactic, this was further evidence that exogenous phytohormone play an important role in the development of phyllotaxy. When the shoot tip with alternate phyllotaxy were used as explants and the concentration of NAA remained unchanging, exogenous 6-BA application could induce a change in phyllotaxy pattern from alternate to opposite. Up to a point, the higher 6-BA concentrations, the higher proportion of opposite phyllotaxy pattern; The pattern of some plants with alternate phyllotaxy had changed in the later period, the higher 6-BA concentrations, the lower transformational nodes of phyllotaxy pattern from alternate to opposite. Without any exogenous phytohormone in rooting medium, the alternate leaf primordium would come back to the original phyllotactic, this was further evidence that exogenous phytohormone play an important role in the development of phyllotaxy.3.The cuttage breeding for the stem segments of Anthirrhinum majus were taken in different treatment to study the phyllotactic development feature of its offspring. The result showed that, without exogenous phytohormone, the phyllotactic pattern of fresh buds came back to the original phyllotactic pattern regardless of any phyllotactic pattern of cuttings. This indicated that the phyllotactic pattern of cuttings remained unchanged by vegetative propagation. The cuttings were treated with different concentration of 6-BA,the higher 6-BA concentrations, the higher transformational nodes of phyllotaxy pattern from alternate to opposite and the lower nodes of flowering; While the cuttings were treated with different concentration of GA3, the higher GA3 concentrations, the higher transformational nodes of phyllotaxy pattern from alternate to opposite and the higher nodes of flowering.When the concentration of GA3 reached a high value, the plants would not blossom. The results showed that the exogenous phytohormone played an important role to its phyllotactic development of the cuttings. And the exeperiment also showed that 6-BA was helpful to the stem diameter thicker and played a positive role in promoting floral bud differentiation; GA3 was helpful to elongation growth of the stem and had an adverse effect on floral bud differentiation.