Effects Of Tributyrin On Production Performance, Intestinal Development In Broilers

The present study was carried out to evaluate the influence of tributyrin on intestinal morphology, caecum microflora, production performance, slaughter performance and meat quality of broiler. This paper aims to study tributyrin to determine the effects of broiler production; according to the different production purposes, decide tributyrin added time; clarify mechanism of tributyrin to promote absorption, improve intestinal health; contrast bacitracin zinc + colistin sulfate and tributyrin action mode.A study using 1080 d-old Arbor Acres broilers was conducted to assess the effect of Pro Phorce SR130 on Performance and intestine histology in broiler. The birds were housed in individual cages for six dietary treatments with 9 replications of 20 broilers each diet. Dietary treatments were: T1, Negative; T2, Positive, Negative + 20 mg/kg bacitracin and 5 mg/kg colistin sulphate; T3, Positive + 500 mg/kg SR in starter diet; T4, Positive + 500 mg/kg tributyrin in both starter and finisher diet; T5, Negative +500 mg/kg tributyrin in starter diet; T6, Negative + 500 mg/kg tributyrin in both starter and finisher diet. Broilers were fed starter diet from d1 to d21 and finisher diet from d22 to d42.At d-14 and d-28 of tested period, 9 broilers were randomly selected from each treatment to determine intestine histology. The results show that: tributyrin(TB, 500 mg/kg) had no significant effect on intestinal p H value at d-14 and d-28(P > 0.05); while adding tributyrin and BZ + CS(20 mg/kg bacitracin zinc +5 mg/kg colistin sulphate) had trend to reduce the duodenum p H value on broilers at d-28(0.05 < P < 0.10). Supplementation of tributyrin to broilers diet significantly increased VH(P < 0.05), reduced CD(P < 0.05) in duodenum and jejunum, increased VH/CD(P < 0.05) in duodenum, tended to have a higher VH/CD(0.05 < P < 0.10) in jejunum, at d-14 of tested period. Broilers fed diet supplemented with antibiotics had a lower VH(P < 0.05) and higher CD(P < 0.05) and lower VH/CD(P < 0.05) in duodenum at d-14 of tested period. Broilers fed diet supplemented with tributyrin had a higher VH(P < 0.05) in duodenum, jejunum and ileum, lower CD(P < 0.05) in ileum, and higher VH/CD in jejunum(P < 0.05) and ileum(P < 0.001), at d-28 of tested period. No significant differences were observed in the intestine histology of broilers at d-14 and d-28 of tested period whether tributyrin supplemented to the starter or the whole experimental period diets. However, supplementation of antibiotics to broilers diet containing tributyrin had a higher VH in duodenum(P < 0.001) and jejunum(P < 0.05), higher VH/CD in duodenum(P < 0.001) and jejunum(0.05 < P < 0.10), lower CD in duodenum(P < 0.05) at d-14 of tested period, and higher VH in jejunum(P < 0.001) at d-28 of tested period. Supplementation of tributyrin to broilers diet significantly reduced Salmonella in the whole experimental period diets. Supplementation of tributyrin to broilers diet in the entire period of the experiment significantly reduced Salmonella(P < 0.05), increased Lactobacillus(P < 0.05) than the phase 1 at d-28 of tested period. Supplementation of antibiotics to broilers diet containing tributyrin had a lower Salmonella(P < 0.05) at d-14 of tested period, and lower E. coli(P < 0.05), Lactobacillus(P < 0.05) at d-28 of tested period.During the feeding trial recorded and calculated the intake, daily gain, feed conversion ratio of broilers. At d-28 of tested period, 9 broilers were randomly selected from each treatment to determine slaughter performance, meat quality and fatty acids. The results show that: supplementation of tributyrin to broilers diet significantly reduced F/G in either phase(P < 0.05) or the entire period of the experiment(P < 0.01),increased ADFI(P < 0.05) during 22~42 d. However, supplementation of antibiotics to broilers diet containing tributyrin had a higher ADG(P < 0.05) in the starter phase. No significant differences were observed in the slaughter performance of broilers at d-42 of tested period whether tributyrin supplemented to the starter or the whole experimental period diets(P > 0.05). Tributyrin can significantly reduce the 42-day-old broilers breast muscle L* value(P < 0.05). Supplementation of tributyrin had a higher leg muscle a* value in the whole phase(P < 0.05). Supplementation of antibiotics to broilers diet containing tributyrin had a higher leg muscle b* value(P < 0.05). Whether adding BZ + CS and tributyrin or not on broiler chest, leg cooked meat ratio, shear muscle, fat content were not significantly different. Tributyrin had no significant effect on chest and abdominal fat, monounsaturated fatty acid content of polyunsaturated fatty acids and saturated fatty acids(P > 0.05).In conclusion, addition of 500 mg/kg tributyrin could improving intestinal development, intestinal health, production performance, and couldn't effect slaughter performance and meat quality of broilers.