| In this thesis, the effects of temperature and starvation stress on morphology, skeletal muscle fibers and blood physiological and biochemical index of Monopterus albus were investigated by artificial temperature and feeding control manner.1. Effect of different temperatures on the morphology, skeletal muscle fibers and hematology of Monopterus albus.A stress trial was conducted to investigate the effects of different temperatures(5 ?,15 ?,25 ? and 35 ?) on Monopterus albus(body weight 40.13 g ± 5.26 g) for two weeks. Monopterus albus does not eat under 15 ? conditions, all treatment groups did not feed in this experiment. Researches showed that the fatness and viscerasomatic index under different temperatures stress were no significant difference. The effects of different temperatures stress on hepatosomatic index significantly(P < 0.05). Blood physiological index of each treatment group had no significant difference. Serum biochemical indicators were affected by temperature. Albumin(ALB) decreased an extremely significant difference at different temperatures(P < 0.01). Glucose(Glu) levels in 35 ? group were extremely significantly higher compared with the control group(P <0.05). High-density lipoprotein(HDL) levels showed an extremely significant difference with control temperature(P < 0.05). The gaps of skeletal muscle fibers also became larger at low temperatures.2. Effect of satvration and refeeding on the morphology and hematology of Monopterus albus.The starvation and feeding experiment in Monopterus albus was carried out at 25 ?.The tested fish were divided into three experimental groups: group A has been starved and began satiation feeding, group B were derivedfood for the same preiod, Group C fed as a control group. Fish sampled at 3, 7, 14, 21, 28, 42 and 56 days. The results showed that:the three sets of liver and other internal organs weight were changed with the variety of body weight and maintained a synchronized pace. The visceral weight of Group A gained faster than group C. Starvation stress impacted on blood physiological index had no significant difference in Monopterus albus(P > 0.05), while the serum albumin were significantly lower than the normal group(P < 0.05). Glucose, cholesterol and triglyceride levels had a decreasing trend under starvation stress. Aminotransferase, high density lipoprotein and blood urea nitrogen levels were no significant change in the starvation group(P > 0.05).3. Effect of low temperature and starvation stress on morphology and skeletal muscle of Monopterus albus.The Monopterus albus were divided into three treatment conditions: 25 ? feeding,25 ?starvation and 5 ?starvation, then divided into five gradient weights: 10 g, 25 g, 40 g,55g and 70 g group, after two weeks until stabilized. The morphology index showed that low temperature and starvation caused a significant impact on Monopterus albus(P < 0.05).However, the hepatosomatic index and viscerasomatic index between different weight groups of had no significant difference(P > 0.05). Fatness presented with a trend of weight gain and increasing, skeletal muscle fibers size showed the same tendency.4. Cloning and sequencing of the muscle-related genes MSTN.The sequences of myostatin gene and its promoter in Monopterus albus were cloned through RT-PCR and genome walking method. The full-length of 1593 bp cDNA including a 1131 bpopen reading frame(ORF) encoding 376 amino acids peptide, a 104 bp 5'untranslated region(5'- UTR) and a 358 bp 3 'untranslated region(3'- UTR). The MSTN gene consists of three exons and two introns, the two introns were 355 bp and 745 bp. The upstream regulatory region of MSTN was more than 2000 bp, which contains a plurality of E-box sequence(CANNTG) and two TATA box. Monopterus albus MSTN gene has a closer phylogenetic relationship with other fish.MSTN was expressed in brain, heart, liver,spleen, kidney, muscle, intestine, testis, ovary and blood and expressed at highest level in muscle. |
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