The Research Of Biochemical Fulvic Acid Bacteria Production Technology And It's Application Effect

We conducted the breeding of produced of Biochemical fulvic acid Strain,and then optimized the fermentation conditions on the amount of nitrogen addition,inoculation quantity,moisture content of materials,fermentation time.We conducted field trials to test the function of the fermentation product,and provided a basis for further research to promote the biochemical fulvic acid agents by using of the semi-solid fermentation products and compared with the existing fulvic acid fertilizer in the market.The results are as follows:1 Five strains were isolated from humus layer collected from forest soil at Xinyang City,Henan Province.Then we cultured and screened several better strains as follows JX1,JX3;JX2,JX6,JX7.The strain JX6 was the best,with the production of fulvic acid content of 20.91%,increasing by 15.52% compared with CK.And there were significant differences compared with others at the level of(P <0.05).Secondly,the content of fulvic acid vaccination strain JX2 product reached 13.65%,accompanying with increasing by 8.14 % compared with the CK.2 Through the research of the optimization of the fermentation process of five strains,then learn that,in this experiment,in terms of the best amount of nitrogen addition,3% urea was the best amount for JX1,JX3;3% urea was the best amount for JX2,JX6,JX7;in terms of inoculum size,3% inoculation amount was the best inoculation amount;in terms of water content of fermentation substrate,50% water content was the optimum fermentation substrate water content for JX1,60% water content was the optimum fermentation substrate water content for JX2,JX6,JX7,70% water content was the optimum fermentation substrate water content for JX3;in terms of fermentation time,25 days was the optimum fermentation time of each strain.3 At maturity stage,application of fulvic acid fertilizer and fermentation product of JX2,JX6,JX7 could significantly increased the content of soil available phosphorus.Compared with control,the content of available phosphorus in JX2,JX6,JX7 and fulvic acid fertilizer was increased by 15.3%~32.5%.There was no significantly difference between fulvic acid fertilizer and JX2,JX6,JX7 treatment available phosphorus content.The fermentation of JX2 increased the content of available phosphorus by 6.5% compared to commercial fulvic acid fertilizer.4 At maturity stage,application of fulvic acid fertilizer and fermentation product of JX1,JX7 could significantly increase the content of soil available potassium.Compared with control,the content of available potassium in JX1,JX7 and fulvic acid fertilizer was increased by 9.4%~20.4%.The fermentation of JX1 and JX7 increased the content of available potassium by 7.4% and 10.1%,respectively compared to commercial fulvic acid fertilizer,non-significantly.5 The difference in each treatment was not significant on plant height.Plant height of each treatment was increased compared with the control.Plant height of JX1,JX2,JX3,JX6,JX7 and fulvic acid fertilizer was increased by 1.4%~3.0% compared with control.The SPAD value of each treatment was increased by 1.5%~5.2%.6 Application of fulvic acid fertilizer and various fermented products significantly increased corn yield by 8.6%~14.2%.The yield of fulvic acid fertilizer between JX1,JX2,JX3,JX7 was no significant differences.7 Applying fulvic acid fertilizer and various fermentation products could increase total dry weight of maize,at maturity stage,compared with control,the total dry weight of JX1,JX2,JX3 JX7 and fulvic acid fertilizer treatment was significantly increased by 23.5%~39.5%.There was no significant differencr between JX1 and commercial fulvic acid fertilizer.8 At the 5-leaf stage,compared with control,the nitrogen accumulation amount in the aboveground plant between JX1 and JX3 reached significant level.At large bell stage,compared with control,the shoot N accumulation amount of fulvic acid fertilizer,JX2,JX6,JX7 treatment significantly increased by 14.1%~15.9%;at mature stage,compared with control,the shoot N accumulation amount of fulvic acid fertilizer,JX2,JX6 treatment significantly increased by 8.7%~14.9%.9 At the 5-leaf stage,fulvic acid fertilizer and JX6 treatment could significantly increased the shoot phosphorus accumulation compared with control.At large bell stage,the amount of accumulation of aerial part phosphorus of fulvic acid fertilizer treatment significantly increased by 27.4% compared with control;aboveground phosphorus accumulation of JX1,JX3 significantly increased by 22.8%,24.4% respectively,compared with control-treated,but there was no significant difference compared with fulvic acid fertilizer.At mature stage,P accumulation amount of aerial part of fulvic acid fertilizer and JX6 treatment significantly increased by 17.4%,16.0%respectively compared with control,but there was no significantly difference between fulvic acid fertilizer and JX6 treatment.All the analysis showed that the ability of fermentation production of biochemical fulvic acid of the strains screened in the present study was outstanding,the results of field trials showed that the fermentation product could increase soil available phosphorus and potassium amount,which led the increasing aboveground biomass,nutrient content and crop yield.Compared to commercial fulvic acid fertilizer,the fermentation of JX2 increased the content of available phosphorus by 6.5% and the fermentation of JX1,JX7 increased the content of available potassium by 7.4% and 10.1%,respectively,which showed that the fulvic acid production strains we screened from this study had a very good application prospect.