| Pseudorabies virus(PRV,PR)is an acute infectious disease caused by porcine pseudorabies virus(PRV virus(PRV),can cause a variety of animal disease,the clinical pregnant sow early abortion,stillbirth,mummy and respiratory system pro clinic symptoms characteristic,the pig is PRV infection sources and natural reservoir host.After infection,the clinical features of fever,neurological symptoms and high mortality,respiratory symptoms in large and medium pigs were shown.By the end of 2011,our country some of the pig continued outbreak and epidemic of swine pseudorabies,leading to neurological symptoms occurred in large numbers of sows miscarriage,newborn piglets dying in large numbers,causing major economic losses.In order to fully understand the epidemic situation of swine pseudorabies in parts of Henan Province,this study from the serum epidemiology and pathogen epidemiology study and molecular epidemiology of the disease epidemic situation of our province in the investigation and analysis,and statistical analysis of pseudo rabies,popular all kinds of risk factors and the effective prevention and control of the disease provide clinical guidance.By enzyme-linked immunosorbent assay(ELISA)in 3711 samples of serum samples from 337 pig farms in January 2015 ~2015 year in December in Henan Province in some areas were detected,PRV g E antibody showed that 337 pig farms in 293 PRV wild virus positive,the total positive rate was 86.89%;the positive rate of pig farm five in the East,Western and southern Henan,Henan,Henan were 81.25%,79.31%,79.31%,85.71%,97.14%;the positive rate of small and medium scale and large-scale pig farms were 85.45%,90.90% and 87.10%;3711 serum samples of the total positive rate was 60.3%,the positive rate of serum samples of sows,boars,pigs were 60.1%,72.6%,57.09%;the results showed that: Henan province PRV wild virus infection is more serious,especially in the area of Henan,Henan;pig PRV virus infection in different stages of production are Seriously,especially the group of boars.By the method of PCR in January 2015 2015 to December 2004 in Henan Province 72 suspected infected pig inspection of 141 tissue disease material for detection of PRV pathogen.The results showed that: 141 samples have 15 samples for PRV virulent pathogen positive,the total positive rate was 10.63%;of which 1-3 months,between April and June,July to September,10-12 month farms detection rate was 25%,by 10.5%,15.79%,10.7%,sample detection rate of 17.85%,5.26%,15% and 5.6%,respectively.Results show that: pig farms in the wild virus infection with the change in the have some differences.In order to understand the part of Henan Province since 2011 status of porcine pseudorabies virus(PRV)epidemic strains and molecular biological characteristics and genetic evolution,this experiment collected parts of Henan 9 strains of PRV isolates,and genetic variation analysis of the g E gene.The results showed that 9 strains of the g E Gene of PRV isolates and other reference strains corresponding sequence has relatively strict conservative.Between these strains between nuclear thatch acid and other reference strains of nucleotide sequence homology respectively 99.7%-100% and 99.1%-99.9%.Multiple amino acid sequence alignment showed that 9 strains of PRV separation plant amino acid homology is 100% with reference strains of amino acid sequence of in 98.1%-99.6%.In one locus occurs the unique amino acid mutations,namely the two amino acids(with ZM Henan2013 and Yangshan(Korea-1996)strains of)by K mutation for f.This is reported for the first time in China GE gene in the position occurred amino acid mutation strains.The relationship between these novel amino acid mutations and PRV pathogenicity and their biological characteristics need to be further studied.In this study,we in China in 2005 and 2015 nearly eleven years of pig pseudorabies serum epidemic learning statistical summary and Ge gene variations of that survey data showed that in the year 3732 relates to the pig farms have 2189 positive field,the positive rate of 58.65%.A total of 272144 sera,which 79754 positive and serum positive rate was 29.31%.The stages of pigs by wild investigation that sows,gilts,commercial pigs,boars,piglets,weanling pig average positive rate 29.66%,20.79%,20.62%,32.99%,23.38%,21.83% respectively.Throughout the investigation results show that China and South Africa,in the northeast,North China,East China,northwest,southwest,Henan area scale pig farm PR wild virus positive rate averaged 22.23% and 27.74,34.53% and 26.3%,24.89%,23.89% and 34.85%.This study has a certain degree of understanding of the incidence of-2015 in China in 2005,and provides guidance for the effective prevention and control of swine pseudo rabies.This study prompted by epidemiological risk factors associated with acquiring swine without quarantine,disinfection is not strict,all in full,without immunization with PR more than 6 kinds of swine diseases,no bird,rodent control measures proposed farm strengthening feeding management and good bio security measures,strict implementation of routine immunization,as well as important disease prevention and control,regular detection and isolation of infected pigs,out of control and PR strategy of positive pigs.Prevention and control results show that the prevention and control program of this study has a very practical value,and provides a reference for the effective prevention and control of PR. |
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