The Parasite Investigation And Animal Ascaris Pcox1 Sequencing Analysis Of Changsha Ecological Zoo

The study in July 2015 is a parasite survey based on the vast majority of animals' feces in the Changsha ecology zoo. It has collected 5 ascaris which has been collected from northeast tiger, white lion, leopard, brown bear (bear) and zebra.Using PCR technology to ascaris mitochondrial sequencing coxl augmentation, reoccupy DNAstar7.1 compare analysis, determine the relationship between within species of 5 samples of the ascaris?This study has practical value in the wild animal disease-control, and contribute basic data for wild animal parasite research?prevention and control.The results are as follows:1. The study has collected the feces samples of 77 kinds of wild animals, among them, 27 samples has been infected by parasite, and the infection rate is 35.5%.2. The infection rate of these feces samples are bleow:Sphaerozoum fuscum is 2.6%; Ascaris lumbricoides is 9.2%,nematode is 43.4%; Trichuris trichura is 10.5%, trematoda is 5.3%.3. According to the above, the infectivity of these parasite are 1-392,1-894,1-280,1-7,1-353,188-298 in turn.4.As a result, the molecular identification of Ascaris lumbricoides is agreed with its Morphological markers:Mitochondrial DNA coxl gene nucleotide sequence analysis results showed that Pcoxl sequence differences in smaller, more obvious interspecific differences. So come to the conclusion that Pcoxl gene sequence can be used as a wild animal genetic markers of the ascaris.5. The result according to the system analysis of 5 kinds of wild animals'mitochondrial is below:The northeast tiger has been infected by Toxocara cati; The white lion and leopard have been infected by Toxascaris leonina; The brown bear has been infected by Baylisascaris transfuga; The zebra has been infected by Parascaris univalens.6. The coxl gene sequencing of 5 wild animal's parasite's mitochondriaResults showed that the results showed that 5 kinds of samples of animal ascaris Pcoxl gene sequence similarity were over 98%,5 kinds of samples of animal worm and other worm in the GenBank corresponding sequence similarity were lower than 92%. Samples of 5 kinds of animal worm species differences (8.2%?14.6%) is more than 5 kinds of samples of animal worm intraspecific variation (0?2.0%), explain Pcox1 provide genetic markers for genetic variation between research, can be used in the samples of 5 kinds of animal ascaris species identification test.