Studies On The Experssion And Fuction Of ?-catenin In The Different Cashmere Growth Periods Of Cashmere Goats

In this research,the number of 12 Liaoning cashmere goats were chosen,the conditions of they were selected that adulthood?healthly and so on.Collection of cashmere goat skin samples of the period that included the Catagen(February),the Telogen(April),the Proanagen(August)and the Anagen(November).Using Relative fluorescence quantitative PCR method to measure the expression of gene of ?-catenin in skin tissue;using Western-blot and immunohistochemistry method to detect expression changes in skin tissue of ?-catenin protein.The growth and morphological changes of hair follicles were measured by in vitro hair follicle culture test,and through endogenous activation ?-catenin signaling pathway.In addition,it was measured that the expression of ?-catenin.To investigated the role of ?-catenin in producing goat hair growth.The results showed that:(1)In the skin tissue,?-catenin gene has expressed in four periods.the highest expression of Anagen(November)(5.5 8±0.13),the lowest expression of the Telogen(April),the expression of Catagen(February)was l,the expression of Telogen(April)was(3.08±0.076).In this periods,there were very signinficant(P<0.01).(2)The results of immunohistochemistry showed that the expression of ?-catenin in primary and secondary follicles of Liaoning Cashmere Goat at different stages.The primary follicles were mainly expressed in the hair matrix,outer root sheath.In the secondary follicles,mainly expressed in the outer root sheath,the level of expression was the highest at November,low at April,expressions of August and February are between November and April in both two kinds of hair follicles.(3)The detection results show that the Western-blot,The protein expression of(3-catenin,the expression level of the Telogen(April)was the lowest,the expression level of the Anagen(November)was the highest,the Catagen(February)and the Proanagen(August)between Anagen(November)and the Telogen(April).(4)The results showed that:The expression level of gene of ?-catenin and the hair follicle's growth length were significantly higher(P<0.01)than the control group in the 2 to 10 mmol·L-1 LiCl groups.The shape of hair follicles maintain good in this group.The expression level of ?-catenin and the hair follicle's growth length were significantly lower than the control group in the 20 mmol·L-1 LiCl group,the hair follicle's growth length were inhibited in vitro.In conclusion:(1)?-catenin was regularly expressed,with the growth of villus,the amount of expresstion of ?-catenin increased gradually;the process by which the gene of ?-catenin participate in the growth of villus.(2)The expression of ?-catenin in primary and secondary follicles of Liaoning Cashmere Goats at different stages.It has been proved that ?-catenin can promote the development of hair follicle during development.(3)The adjunction of 2 to10 mmol·L-1LiCl can promote hair follicle growth and the level of ?-catenin mRNAs' expression,in which 5 mmol·L-1 has the best effect.