Effects Of Different Dosage Of Licorice Feed On Characteristic Flavor Compounds In Multi Wave Mutton

The study aimed to study the effects of different proportions of Glycyrrhiza uralensis Fisch on volatile substances in subcutaneous fat of Duolong sheep under the condition of alkaline environment in Xinjiang.Two pretreatment methods,headspace solid phase microextraction and simultaneous distillation extraction,were used to detect volatile components in subcutaneous fat of Duolong sheep using gas chromatography-mass spectrometry.A method for extraction and determination of volatile components in subcutaneous fat of Duolong sheep has been established.The effects of different amounts of liquorice on volatile components in diets were studied.The main results were as follows:Firstly.The extraction conditions of volatile compounds in subcutaneous fat of Duolang sheep were determined by pretreatment with Simultaneous Distillation-Extraction(SDE)method.The results showed that the extraction time was 80 min,the dosage of the sample was 100 g,the extraction solvent was ether and the column was DB-FFAP.The temperature of the column was 40 ?,kept at 2 ?,ascended to 120 ?at 6 ?/min,For 1 min,then to 4 ?/min to 160 ? for 1 min,and finally to 2 ?/min to 210 ? for 5 min.It basically meets the requirements of the study on volatile fatty acids in subcutaneous fat of Duo Lang sheep.Second.The extraction conditions of volatile compounds in subcutaneous fat of Duolang sheep were studied by pretreatment with the headspace solid-phase microextraction(HS-SPME)method.The results showed that the extraction time was 60 min,the extraction temperature was 100 ? and the dosage was 10 g.It basically meets the requirements of the study.Thirdly.Under the method of simultaneous distillation and extraction,the subcutaneous fat of Duolang sheep fed with different amounts of liquorice was analyzed.The results show that when adding 40 %licorice effect on volatile substances is large,part of volatile fatty acids reduced the amount of hexanoic acid 31.75 %,octanoic acid,decanoic acid 19.51 %,12.43 %,34.47 % 4-methyl octanoate,4-methyl pelargonate 41.64 %.Fourthly.The different proportions of licorice were added in the Diet and the extraction conditions of volatile compounds in subcutaneous adipose tissue of Duolang sheep were studied by pretreatment with theheadspace solid-phase microextraction(HS-SPME)method.The results showed that a total of 32 compounds were detected,including acids,esters,aldehydes,ketones and so on,and the compounds had different degrees of variation.Among them,the content of acid compounds which affected the flavor compounds was reduced by 5 % and aldehydes by 3 %.Fifth..A total of 83 compounds were detected in subcutaneous fat of Duolang sheep with the two pre-treatment methods.The three major compounds were acid compounds(40.96%),esters(19.28%)and aldehydes(12.05%).