| ?Background? Currently there are around10%-15%of couples infertility, malefactor accounted for50%and male reproductive capacity showed a trend of apparentdecline in worldwide. Complex causes result in male infertility, fertility related genes isone of the important reasons. Mitochondrion is the only organelle of sperm. The classictheory thought he mitochondria produce ATP to supply sperm energy by oxidativephosphorylation. Although the importance of energy produced mitochondrial to spermmovement and function is still controversial, but we find mitochondria DNA (mtDNA)mutations and deletions in men with abnormal sperm.In recent years, studies of spermmtDNA caused widespread concern and focused on DNA mutations and deletions. Thestudies on DNA copy number is fewer, but some scholars have suggested that we canassess the functional status of sperm mitochondria by measured the mtDNA copynumber.?Objective? The purpose of this study is through comparing mtDNA copy numberand integrity in different quality sperm and found the changes of mtDNA copy numberand integrity in abnormal sperm, and then discusses the influence of sperm mtDNAcopy number and integrity on male fertility.?Methods? Real-time quantitative PCR (Real-time PCR) technology is a matureresearch means with fast, accurate, sensitive characteristics. It is widely used to studynot only the field of molecular biology and cell biology, and can quantitatively detectthe changes in gene expression and copy number of target gene.?Results?52cases of semen samples,30%layer of normal sperm mtDNA copynumber (4.85±3.00) compared to its80%layer (0.80±0.45), the difference wasstatistically significant (P<0.01)?30%layer of asthenozoospermia mtDNA copy number?1.92±1.29?compared to its80%laye?r0.70±0.52?, the difference was statisticallysignificant (P<0.01)?30%layer of Oligozoospermia mtDNA copy number ?13.33± 9.96? compared to its80%layer ?1.52±0.68?, the difference was statisticallysignificant (P<0.01)?30%layer of oligoasthenozoospermia mtDNA copy number?5.34±4.62? compared to its80%layer?1.93±0.65?, the difference was statisticallysignificant (P <0.05)?In the80%layers?A significant increase in mtDNA copy numberobserved in Oligozoospermia?1.52±0.68?and oligoasthenozoospermia?1.93±0.65?compared to the patients with normal semen parameters?0.80±0.45?(P<0.01). and adecrease in mtDNA integrity were observed in oligoasthenozoospermia?9.33±2.71?compared to the patients with normal semen?27.01±17.36??asthenozoospermia?32.36±21.75?? Oligozoospermia?17.63±7.64?(P<0.01). In the30%layers?themtDNA copy number from Oligozoospermia?13.33±9.96?and asthenozoospermia?1.92±1.29?were significantly increase than normal semen samples?4.85±3.00?(P<0.05). By comparing80%layer sperm parameters correlation, we concludedthat mtDNA integrity was positively correlated with sperm count?r=0.5842,P<0.01?,mtDNA copy number was negatively correlated with mtDNA integrity?r=-0.6261,P<0.01?,and mtDNA copy number was negatively correlated with spermcount?r=-0.6345,P<0.01?.?Conclusions? The integrity decreased and copy number increased of mtDNA formpoor sperm samples. We suggest that mtDNA quantity and quality may serve as usefuldiagnostic markers of sperm quality. |
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