| Objective: Pterygium is a prevalent human surface disease that manifests as a fibrovascular conjunctival outgrowth that sometimes can invade the cornea. It is one of the most commonly seen clinical ophthalmic diseases,involving one or both eyes. The pathogenesis of pterygium has not been completely explored. The causes of the occurrence and development of pterygium are complex. It may be the result of one main pathogenic factor or the combined effect of multiple related factors. The ultraviolet radiation has been described as the main cause of pterygium. The change of its morphology can lead to the decrease of the visual acuity due to the irregular astigmatism. Now the excision of the pterygium is the most effective method to treat pterygium; however, recurrence, as one of the complications still can not be avoid. It's necessary to learn the pathogenesis of the pterygium to reduce the incidence of the recurrence. In recent years, there are many studies about the pathogenesis of the pterygium. Our recent research has focused on the relation between the pterygium and some factors such as the ultraviolet radiation exposure, the expression of cell cytokines, excessive cell apoptosis, cell proliferation and so on. Some published data haven shown that pterygium is closely related to the expression of the inflammatory cytokine. This study mainly explains theoccurrence and development of pterygium in terms of the expressions ofinflammatory cytokines, vascular endothelial cell growth factor, andnuclear factor ?B. Interleukin are a group of cytokines that greatly involved in the inflammation process. Many other inflammation cells have been found to contribute in the pterygium as well. Among the angiogenic factors, the vascular endothelial growth factor is the most effective cytokine which can promote the migration of the vascular endothelial cell to complete the form of the new blood vessels.Nuclear factor ?B plays an important role in some physiological processes, such as inflammation cell apoptosis, cell proliferation, angiogenesis, which can regulate the expression of some other cytokines.This study is designed to characterize the pathogenesis of the pterygium from the following two perspectives:1 To compare the expression leves of the IL-6, IL-8, NF-?B, VEGF between the pterygium and normal conjunctiva;2 To study the relevance among the expression of IL-6, IL-8, NF-?B, VEGF.This not only make us have a further study about the pathogenesis of the pterygium, but also make us have a more comprehensive congnition of the treatment of the pterygium. Meanwhile, it provides more effective directly evidence for the operation methods and the postoperative medication.Methods:1 Ten patients were included in the study from June 2015 to December 2015. Of these patients, 5 were males and 5 females, with an agerange of 40-55 years and an course range from 6 months to ten years. All the pterygium selected had no other diseases of cornea and conjunctivas. A complete examination has been underwent to exclude some related diseases such as scleritis and iritis, and systemic dieases which can cause ocular cell proliferation such as tumor. The pterygiumexcised was set up as study group, while its surrounding normal conjunctiva of the same eye as the control group that covers about 2mm×2mm. The specimens were put into the refrigerator of-80? within 10 minutes after they detached from the patients. RT-PCR was conducted to analyze the expression levels of IL-6?IL-8?NF-?B?VEGF, the relation among these cytokine.2 The expression levels of IL-6?IL-8?NF-?B?VEGF of the study group and control group were represented by the relative expression quantity comparing with the GAPDH. The differences between the studygroup and control group were analyzed by paired-samples T-test, and the relationship among the above cytokines with bivariate correlations.3 All could be analyzed by SPSS 13.0 statistical software.Results:1 IL-6 of the experimental group and the control group:Compared with the control group, the experimental group showed higher expression level of the IL-6, with a significant difference between the two groups(P=0.00<0.05).2 IL-8 of the experimental group and the control group:Compared with the control group, the experimental group showed higher expression level of the IL-8, with a significant difference between the two groups(P=0.00<0.05).3 NF-?B of the experimental group and the control group:Compared with the control group, the experimental group showed higher expression level of the NF-?B, with a difference between the two groups(P=0.00<0.05).4 VEGF of the experimental group and the control group:Compared with the control group, the experimental group showed higher expression level of the VEGF, with a significant difference between the two groups(P=0.00<0.05).5 There are correlations among the expression of IL-6?IL-8?NF-?B?VEGF in the experimental group. The correlation coefficient is 0.883 between IL-6 and NF-?B, and 0.827 between IL-8 and NF-?B( P=0.003<0.01). The expression of IL-6 and IL-8 were both correlated with NF-?B expression. While there is no correlations between others factors(P>0.01).Conclusion:1 The increased and abnormal expression leves of IL-6?IL-8?NF-?B?VEGF in the study group indicated the inflammatory nature and angiogenesis characteristic of the pathogenesis and development of pterygium tissues. It also provides a reliable and strong evidence illustrating the pathogenesis of the pterygium.2 The positive correlations between the expression of IL-6?IL-8 and NF-?B in the experimental group indicates the possible revelation between all the other interleukin and NF-?B, which we can reasonably guess. It can also provide some new directions and ideas for further research of the pathogenesis of the pterygium. |
PDF Full Text Request