Study On The Correlation Between Killer Cell Immunoglobulin-like Receptor(KIR),Human Leucocyte Antigen G(HLA-G) And Hypertensive Disorders Complicating Pregnancy

Hypertensive disorders complicating pregnancy(HDCP), a disease peculiar to pregnancy, is a common obstetric complication with symptoms of high blood pressure,proteinuria, fetal growth restriction and so on after twenty gestational weeks. Its pathogenesis is not clear, thus the immunological factors have attracted more and more attention recently. At present most scholars believe that the most important factor is the destruction of immune balance of maternal-fetal interface and the change in immune tolerance, which cause a series of cascading immune responses, including systemic ones.Objective:The thesis aims to discuss the correlation between KIR and HLA-G and hypertensive disorders complicating pregnancy through analyzing peripheral blood KIR level in patients and the expression of HLA- G in placental tissueMaterials and Methods:The author has selected inpatients diagnosed with HDCP from December 2013 to December 2014 in the First People’s Hospital of Jingdezhen, with 30 cases of gestational hypertension, 30 of mild preeclampsia, 30 of severe preeclampsia and 30 of normal pregnancy(control group), all of whom are of the Han Nationality and singleton pregnancy cesarean delivery.(1) 3 milliliters of peripheral venous blood are drawn before delivery and level of KIR in the serum is detected via enzyme-linked immunosorbent assay(ELISA).(2) 2cm×2cm×2cm central placental tissue is taken and sliced 10 minutes after delivery of the placenta. Immunohistochemical technique is used to test the HLA- G expression intensity and positive rate.Results:1.KIR content in the serum of HDCP patients is higher than that of control group(P <0.01) and increases with the aggravation of the disease(P <0.01).2.HLA- G expression intensity and positive rate of HDCP patients are much lower than those of the control group(P < 0.01) and decrease with the aggravation of the disease, the difference marked(P < 0.01).Conclusions:The increase in peripheral blood KIR of HDCP patients can be a reflection of the disease. The HLA- G expression will decrease and reflect the severity of the disease.