Intra-Articular Injection Of Ozone Water Treatment Of Knee Osteoarthritis Clinical Research

ObjectiveThis thesis aims to observe the clinical efficacy of treatment for knee osteoarthritis using ozone water with compound betamethasone injection and intra-articular injection. Compare the differences among various groups by detecting the content of IL-1? and TNF-? in the synovial fluid as well as the Western Ontario and McMahon Lancaster University (WOMAC)osteoarthritis index scores before and after treatment in each group. Then evaluate the effect of the treatment by using ozone injection in osteoarthritis of the knee joint so as to explore its treating mechanism and provide a reliable basis for future clinical treatment.Methods107 patients with knee osteoarthritis were collected and divided into control group and experimental group using a random envelope method. Inject compound betamethasone injection into articular cavity of patients in the control group, while inject a concentration of 20ug/ml ozone water into the test group. Each group were injected once every week, three times for one course. All patients in the two groups were extracted synovial fluid respectively one week before and after the treatment. Detect the content of IL-1? and TNF-? in the synovial fluid by using enzyme-linked immunosorbent assay (ELISA). Then assess the efficacy of the severity of their arthritis patients before and after treatment using the Western Ontario and McMaster Universities (WOMAC) osteoarthritis Yan index.Results1. Before treatment, difference in WOMAC score of the two groups of patients was not statistically significant (P>0.05); After treatment, the difference was statistically significant (P<0.05) as the WOMAC scores were lower than before; WOMAC score of the experimental group was significantly lower than the control group after treatment, which was statistically significant (P<0.05).2. Before treatment, difference in the content of IL-1? in the synovial fluid of two groups of patients was not statistically significant (P>0.05); After treatment, the content of synovial fluid IL-1? levels were lower, which means the difference was statistically significant (P<0.05). No significant difference in content of IL-1? in the synovial fluid of patients from both test group and control group (P>0.05).3. For the two groups of patients, difference in the content of TNF-a in synovial fluid was not statistically significant (P>0.05); After treatment, the content of TNF-a in synovial fluid was lower than before, and the difference was statistically significant (P<0.05), while the content of TNF-? in synovial fluid before and after the test had no significant difference (P>0.05).Conclusion1. The intra-articular injection of ozone water treatment not only can reduce knee osteoarthritis WOMAC score, but also relieve pain significantly, reduce joint symptoms as well as improve people's quality of life;2. The intra-articular injection of ozone water treatment has an effect on reducing the content of IL-1? and TNF-a in joint fluid from the patients with knee osteoarthritis and it also can reduce inflammation in the joints.