Clinical Observation Of Human Rhinovirus Infection Rate In Children With Acute Asthma

Objective We investigate the infection of the human rhinovirus(HRV)in children with asthma,and the concentration of serum intercellular cell adhesion molecule-1(ICAM-1),which is the receptor of HRV.Then we explore the pathogenesis of asthma caused by HRV.Our study provide evidence for the etiology and pathogenesis of asthma caused by HRV,and has high guidance value on the classification of HRV antigen level.Method By convenience sampling,92 children with acute exacerbation of asthma were collected in October 2015 to April 2016 in our pediatric department who received hospitalization as the experimental group.30 children,without anaphylactic disease or parasitization history and not using glucocorticosteroid or immunomodulator within 6 months were selected as the control group.The ELISA method was adopted to test the HRV level in serum.The experimental group was divided into HRV positive group and HRV negative group based on HRV level.The ELISA method was applied to test serum ICAM-1 concentration in experimental group and serum HRV concentration in HRV positive group.Results HRV was positive in 64 children of experimental group(the infection rate: 69.57%),and HRV was negative in 28 children(the non-infection rate: 30.43%).There was no case of HRV positive in control group.The infection rate of experimental group was significantly higher than that of control group(P<0.01).Comparison of clinical symptoms of the experimental group of HRV positive and HRV negative,HRV positive patients with fever,cough,wheezing,nasal congestion,runny nose,breathing difficulties and other clinical manifestation ratio was significantly higher than that of HRV negative group.HRV positive and HRV negative blood white blood cell count in the experimental group(df=12.731,t=7.298,P=0.000),P<0.05,the difference was statistically significant.Neutrophil ratio(df=0.015,t=16.808,P=0.000),P<0.05,the difference was statistically significant.C-reactive protein(C-Reactive protein,CRP)level(df=6.246,t=8.406,P=0.000,P<0.05),the difference was statistically significant.Comparison of creatine kinase isoenzyme(CK-MB)levels(df=12.308,t=17.713,P=0.000),P<0.05,the difference was statistically significant.The average hospitalization days(d)were compared(df=8.896,t=4.927,P=0.000),P<0.05,the difference was statistically significant.The concentration of ICAM-1 in HRV negative group was(109.12 + 117.4)ng/L,while the concentration of ICAM-1 in HRV positive group was(229.9 + 314.52)ng/L,there was a significant difference between the two groups(Z=-4.936,P<0.01).In the HRV positive group in children with asthma,the concentration of HRV high expression level in the ICAM-1 group was(1634.468 + 2179.925)ng/L,significantly higher than the low expression of HRV concentration in ICAM-1 group(113.266 + 121.36)ng/L,the difference was statistically significant(Z,=-102.858,P<0.01).Conclusions HRV infection rate in children with acute exacerbation of asthma was significantly higher than the healthy children.The levels of neutrophils,C-reactive protein(CRP)and creatine kinase isoenzyme(CK-MB)in children with HRV-positive asthma were higher than those in children with HRV-negative asthma.Rhinovirus infection in children with asthma caused by the average number of days of hospitalization has improved.ICAM-1 concentration in HRV positive group was higher than the HRV negative group.As the increasing the HRV concentration,the ICAM-1 concentration increasing spontaneously,which indicated HRV promote the expression level of ICAM-1,which may result in the exacerbation of asthma in children.