Experiment Research Of Abdominal Acupuncture In Mice Model Of Chronic Obstructive Pulmonary Disease With Respiratory Muscle Fatigue

Objective:This experiment is to obseve the NADPH Oxidase(NOX),cytochrome oxidase(CCO),succinic dehydrogenase(SDH),tumor necrosis factor-?(TNF-?)in the serum and mitochondria of diaphragmatic muscle tissue in models of chronic obstructive pulmonary disease well fatigue.As the breakthrough point,the experiment through the "abdominal acupuncture therapy" intervention to explore the mechanism with the bdominal needle,in order to promote the application of abdominal acupuncture therapy in lung diseases as well as provide reliable animal experimental evidence.Methods:Selecte 35 SD mice with 180g-220 g and adaptive breeding 7 days.It random drawing 10 mice as the control group,the remained mice made the COPD with respiratory muscle fatigue model.Making model 30 days,randomly selected 5 model mice determination model success.Grouping three group in 20 mice according to number table method and marked as the control group,model group and abdominal acupuncture group with 10 mice in each group.The Model group and abdominal acupuncture group use smudging joint twice lipopolysaccharide method for to buildup of model of COPD,the control group injected saline volume.The cycle is 30 days.Model methods: on day 1 and day 20 injection of lipopolysaccharide in the mice airway within 200 ug(200 ul),the day 2-19 and the day21-30 with passive smoking.In the process of cigarette smoke experiment with once daily and continue 60 minutes,each time with 10 cigarette,once passive smoking interval of at least 4 hours.All rats were raised in cage free drinking water and eat.Smoke in the processof tapping smoke box once every 3 minutes to avoid a cluster in rats.Therapeutic schedule is abdominal acupuncture group is Oxygen therapy and acupuncture treatment for daily basis Zhongwan Point,CV 10 hole,Qihai(CV 6),Guanyuan point,point st 24,Qixue(K13).Model group and the control group are given Oxygen therapy,treatment cycle is 30 days,once week observation rat weight,hair,drinking water feeding,onyx,activity,and so on.30 days obseve the NADPH Oxidase(NOX),cytochrome oxidase(CCO),succinic dehydrogenase(SDH),tumor necrosis factor-?(TNF-?)in the serum and mitochondria of diaphragmatic muscle tissue.After rats shall be put to death,take out the inferior lobe of right lung and sections with paraffin-embedded,obseve the lung histopathology by HE staining in the control group,model group and abdominal acupuncture group of rat,analyse the data.Results:1.Building at the end of 31 days,randomly selected 5 mice observe the general condition of mice,lung tissue under the form to the naked eye,then the lung tissue pathology change,and proof copy success rat model.2.The model mice will be divided into three groups,the control group,model mice and abdominal acupuncture group.Abdominal acupuncture group rats weight,hair,water feeding,onyx,activities and so on compared with model group were increased;3.Abdominal acupuncture group and model group rats TNF-? levels were increased in serum,diaphragmatic muscle tissue compared with the control group(P<0.05),and abdominal acupuncture group of rats was significantly lower than model group(P<0.05);4.Abdominal acupuncture group and model group rats diaphragmatic the NOX,SDH,CCO activity has decreased compared with the control group(P<0.05),abdominal acupuncture group have lower than the model group(P<0.05);5.The lung tissue of pathology with airway epithelial cilia neatly,normal bronchoalveolar structure in the control group;the model group have cell degeneration,necrosis,fall off,goblet cells increased,epithelial cilia disorder,bronchial wall inflammatory hyperemia and edema and a large number of inflammatory cells infiltration in the bronchus wall,as well as alveolar structure disordered,interval shorter,alveolar tissue fusion,alveolar space expanded,as emphysema changes in chronic obstructive pulmonary disease;the abdominal acupuncture grouprats can significantly reduce the bronchial mucosa and submucosa of inflammatory cells infiltration,reduce mucosal epithelial cell necrosis and shedding,reduce the airway secretions.Research conclusion:COPD with respiratory muscle fatigue mice serum and diaphragmatic muscle tissueTNF-? levels higher than the control group,and the contents mice abdominal acupuncture rise significantly lower than that of model group.The NOX,SDH,CCO has reduced compared with the control group.The lung tissue of pathology in bronchial mucosal epithelial degeneration,necrosis,fall off,bronchial wall appear a large number of inflammatory cells infiltration as well as lots of effusion.It have reduced after abdominal acupuncture treatment.It proved that the abdominal acupuncture treatment have significant efficacy by lowering the serum of rats and the diaphragmatic muscle TNF-? levels,improve the diaphragm mitochondrial NOX,SDH,CCO,as well as reducing the airway inflammation,improve the respiratory muscle endurance,in order to prevent deterioration of COPD.This experiment aim to approach abdominal acupuncture stating the mechanisms of COPD with respiratory muscle fatigue by means of the inflammatory mechanism as well as cells,biochemica.Finalil to promote the application of abdominal acupuncture therapy in lung diseases as well as provide reliable animal experimental evidence.