Construction Of Nano Soybean Meal Based Nutritional Delivery System Of Vitamin B2 And Vitamin B6

Vitamin B₂,also known as riboflavin,forms flavin mononucleotide?FMN?and flavin adenine dinucleotide?FAD?in the body,acting as coenzyme.The two coenzymes play an important role in energy metabolism,especially in the metabolism of fat,carbohydrate and protein.Vitamin B₆ including pyridoxine,pyridoxal and pyridoxamine,plays an important role in physiological activity metabolic reactions in human and animal body.In addition,vitamin B₆ is also involved in the metabolism of fat,reducing the cholesterol in the blood.Vitamin B₂ and cooperated with vitamin B₆ participates in some metabolic process in the body,such as amino acid metabolism.However,vitamin B₂ and vitamin B₆ are easily destroyed by light and alkaline enviroment,causing the damage of their chemical structure and reduction of bioactivity which is unfavorable for their application.Soybean meal?SBM?is an ideal food material rich of protein.In this study,SBM through superfine pulverizing firstly and ball mill grinding secondly turn into the nSBM,that readily aggregates into larger particles.It provides a new possibility of the use of vitamin B₂ and vitamin B₆ in the solution.In this study,our work included constructing the delivery system and the characterization of the delivery system.Then,we used CCK-8 to detect the cytotoxicity of nSBM on Caco-2 cells and the bioavailability of vitamin B₂ and vitamin B₆ base Caco-2 monolayer.First work of this study is the quantification of vitamin B₂ and vitamin B₆ in a mixed solution at a high concentration by fluorescence spectrophotometry.Firstly,the determine wavelength of vitamin B₂ and vitamin B₆ was Ex/Em=480/530 nm and Ex/Em=300/400 nm respectively.A mathematical model was established to calculate the actual concentration of vitamin B₆ based on the apparent concentrations of vitamin B₆ and vitamin B₂,which were quantified directly when vitamin B₂ and vitamin B₆ were mixed together and R2 is 0.999.By ANOVA,different particle sizes of SBM had significant impact on the loading efficiency of vitamin B₆,but no significant influence on vitamin B₂.Loading efficiency of vitamin B₂ related to the amount of nSBM and vitamin B₂,regardless of the amount of vitamin B₆.To the contrary,not only the amount of nSBM and vitamin B₆ but also the vitamin B₂ influenced the loading efficiency of vitamin B₆.The release of vitamin B₂ in delivery system within 5h were 86.153%,89.585% and 97.761% corresponding the particle size of nSBM 200 nm,500nm,800 nm.However,as for vitamin B₆,the release in delivery system within 5h according to the particle size of nSBM were 93.691%,91.741% and 75.941%,respectively.Comparing the IR spectra of vitamin B₂ vitamin B₆,and nSBM with delivery system,a new characteristic peak?3431.55cm-1/3420.38cm-1?was found,which meant that the hydrogen bond was the major binding force between vitamin B₂ or vitamin B₆ with nSBM.SEM results show that the nSBM particle turned to spheroidal particle and the particle size was 2 ?m.In CCK-8 detection,cell viability value over 100%,suggesting that nSBM had no effect on Caco-2 cells.The transport of vitamin B₂ and vitamin B₆ was studied by Caco-2 monolayer cell model.There was no significant difference between the delivery system and free vitamin B₂ and vitamin B₆.Considered that delivery system could stay longer in the intestinal tract,there were higher bioavailabilities of vitamin B₂ and vitamin B₆ were higher in delivery system.This study used nSBM to delivery vitamin B₂ and vitamin B₆ which could enhance the bioavailability of vitamin B₂ and vitamin B₆.The application of food borne materials is the main innovation of this study.Smaller nSBM particle should be produced with expect to increase the loading efficiency of vitamin B₂ and vitamin B₆,enhance the stability of the delivery system need to be invested in future work.