Expression And Correlation Analysis Of Sox2,Sall4,Klf4 And β-catenin In Endometrial Adenocarcinoma

Objective: 1.To investigate the expression and clinical significances of Sox2,Sall4,Klf4,β-catenin in different endometrial tissues,and analyze their correlation of expressing in endometrial adenocarcinoma tissue samples.2.To explore the relationship between Sox2,Sall4,Klf4 and β-catenin expression level and clinicopathological features in endometrioid adenocarcinoma,which provides a better theoretical basis for early diagnosis,treatment and prognosis monitoring of endometrioid adenocarcinoma.3.To explore Paclitaxel on endometrial adenocarcinoma’s chemotherapy effect through observes the expression of Sox2,Sall4,Klf4 and β-catenin before and after treatment of paclitaxel in Ishikawa cell and HEC-1A cell.And provide the theoretical basis for the further targeted therapy of the tumor.Method: 1.140 cases of endometrial tissues were collected,including 30 cases of normal proliferative endometrium,40 cases of endometrial hyperplasia(not with atypical hyperplasia in 20 cases and atypical hyperplasia in 20 cases),70 cases of endometrioid adenocarcinoma(30 cases of well-differentiated carcinoma,20 cases of moderately and poorly differentiated carcinoma).The expressions of Sox2,Sall4,Klf4 and β-catenin were detected by immunohistochemical method MaxVisionTM.2.CCK-8 was used to detect the proliferation inhibition rate of Ishikawa and HEC-1A cell with different concentrations of paclitaxel.3.The expressions of Sox2,Sall4,Klf4 and β-catenin were detected by Western blot before and after drug treatment,and the effect of paclitaxel on endometrioid adenocarcinoma was studied.4.Statistical methods: SPSS19.0 software for statistical analysis of experimental data,and P<0.05 for the statistical difference.Result: 1.Sox2,Sall4 and Klf4 were not expressed in normal endometrium tissues and endometrial hyperplasia tissues,but the expression of β-catenin was higher in the membrane of endometrial glands cell.The expressions of Sox2,Sall4,and Klf4 in normal endometrium tissues and endometrial hyperplasia tissues were no significance(P>0.05).The expressions of Sox2,Sall4 and Klf4 in endometrial carcinoma were higher than that in normal endometrium tissues and endometrial hyperplasia tissues,and the expression of β-catenin was decreased(all P<0.05).2.The expression of Sox2,Sall4,Klf4 and β-catenin was related to the histological grade,depth of invasion and lymph node metastasis of endometrioid adenocarcinoma(P<0.05),but not to the patient’s age(P>0.05).3.There was a negative correlation between the expression of Sox2,Sall4,Klf4 and β-catenin in endometrioid adenocarcinoma(β-catenin and Sox2: r =-0.685,P<0.05;β-catenin and Sall4: r=-0.453,P<0.05;β-catenin and Klf4: r=-0.438,P<0.05).Among expression of Sox2,Sall4 and Klf4 were positively correlated with each other,respectively(Sox2 and Sall4: r = 0.453,P<0.05;Sox2 and Klf4: r = 0.470,P<0.05;Sall4 and Klf4: r = 0.578,P<0.05).4.At the concentrations of 1μg/ml,5μg/ml,10μg/ml,20μg/ml and 100μg/ml,the inhibitory rate of Ishikawa and HEC-1A cells were significantly inhibited.5.The expression of Sox2,Sall4 and Klf4 in Ishikawa and HEC-1A cells was significantly increased after paclitaxel treatment.The expression of β-catenin was not changed after paclitaxel.Conclusion: 1.The expression of Sox2,Sall4,Klf4 and β-catenin is closely related to endometrioid adenocarcinoma and is a tumor-related factor.2.The expression of Sox2,Sall4,Klf4 and β-catenin is closely related to the clinicopathological factors of endometrioid adenocarcinoma,which suggests that they may be involved in the pathogenesis of endometrioid adenocarcinoma and its expression for the choice of tumor treatment and the prognosis of effective monitoring to provide an effective basis.3.The expression of Sox2,Sall4,Klf4 and β-catenin in endometrioid carcinoma was highly correlated,suggesting that Sox2,Sall4 and Klf4 may interact with CSCs(Cancer stem cells)and may be associated with Wnt/β-catenin signaling pathway,and jointly promote the occurrence and development of endometrioid adenocarcinoma.4.The expression of Sox2,Sall4 and Klf4 was increased,but β-catenin expression level was nothing change,which speculated that cells may develop drug resistance that single drug for the treatment of endometrium adenocarcinoma may not be effective,and easy to appear tumor recurrence and metastasis.