Phatmacy Study Of Jiejiiu Oral Liquid On Gastric Mucosal Lesions And Liver Injury Induced By CCl₄

Objective: To investigate the protective effects of Jiejiu Oral Liquid on liver injury induced by CCl₄ and acute alcohol-induced gastric mucosal Lesions in mice and the mechanism of action,wihch could provide scientific evidence for further study.Methods:1.Protective effects of Jiejiu Oral Liquid on liver injury mice induced by CCl₄This experiment used CCl₄ to build the experimental mice model and mice were randomly divided into 6 groups,each group with 10 mice: control group,model group,positive drug group?Bifendate?,low concentration group of jiu Oral Liquid,middle concentration group of jiu Oral Liquid,High concentration group of jiu Oral Liquid.Low concentration group of jiu Oral Liquid,middle concentration group of jiu Oral Liquid,High concentration group of jiu Oral Liquidwere given 6.25g/kg,12.5g/kg,25g/kg Jiejiu Oral Liquid separately.After 12 h of CCl₄ treatment,the changes of liver index was compared,the damage of liver tissues was observed by light microscope.The fully automatic biochemical analyzer was employed to detect the level of ALT,AST,ALB,GLB,TP,the method of liver tissue homogenate was used to detect the level of MDA,SOD,GSH and their changes were observed.In addition,the expression of Bcl-2,MnSOD and Caspase-3 in liver were observed by the immunohistochemical method.The gene expression level of Nrf2,SOD in liver was observed by using fluorescent PCR detection.All the data were processed by the variance analysis.2.Protective effects of Jiejiu Oral Liquid on acute alcohol-induced gastric mucosal Lesions in mice60 mice were randomly divided into 6 groups,each group with 10 mice:control group,model group,positive drug group?sucralfate?,low concentration group of jiu Oral Liquid,middle concentration group of jiu Oral Liquid,High concentration group of jiu Oral Liquid.Low concentration group of jiu Oral Liquid,middle concentration group of jiu Oral Liquid,High concentration group of jiu Oral Liquidwere given 6.25g/kg,12.5g/kg,25g/kg Jiejiu Oral Liquid separately.All groups were made the model of acute alcohol-induced by drinking 95 bH Ethanol except the normal control group.The effect of Pathological histology and injury of gastric mucosal were observed.The method of mucosal tissue homogenate was used to detect the level of NO,ET-1,TNF-?,IL-6 and PGE2.Results:1.Jiejiu Oral Liquid can decrease the serum content of ALT?AST?P<0.01 or P<0.05?.High concentration group of jiu Oral Liquid can decrease the level of MDA,and increase the activity of SOD and GSH in liver homogenate?P <0.05?.Jiejiu Oral Liquid can reduce the scope and degree of liver tissue necrosis,and reduce inflammatory cells infiltration.by light microscope.High concentration group of jiu Oral Liquid can increase the level of MnSOD? Bcl-2protein expression and decrease the level of Caspase-3 protein expression by the immunohistochemical method.More importantly,Jiejiu Oral Liquid can increase the level of Nrf2 gene expression,thus increased the gene expression of SOD.2.The gastric mucosa of mouse in model group appeared obvious bleeding,erosion,flake ulcer.Compared with the model group,the gastric mucosa of the Jiejiu Oral Liquid therapy group was obviously reduced the acute damage index in mouse?P < 0.05 or P<0.01?.Compared with the model group,high-dosage group and middle-dosage group of the Jiejiu Oral Liquid decreased the content of ET–1 ? TNF-? and IL-6?P<0.05 or P<0.01?.high-dosage group of the Jiejiu Oral Liquid increased the content of NO?P <0.05?.Jiejiu Oral Liquid had no obvious effect on the content of PGE2?P>0.05?.Histopathologic examination revealed Jiejiu Oral Liquid can reduce the shedding of gastric mucosa and inflammatory cells infiltration.Conclusion: For acute carbon tetrachloride liver injury in mice,Jiejiu Oral Liquid have a certain protective effect.Improving liver antioxidant capacity,and inhibiting liver cell apoptosis may be the mechanism of action.Jiejiu Oral Liquid has some certain protective effect on acute alcohol-induced gastric mucosal Lesions in mice at the dose of 12.5g/kg and 25g/kg,which may due to reducing gastric mucosa blood circulation obstacle and the inflammatory injury of gastric mucosa.