| Objectives To observe Tonifying-qi-Nourishing-yin-Activating-blood Decoction(TNAD)regulating glucolipid metabolism and affecting expression of micro RNA-29 in adipose tissue of type 2 diabetes mellitus rat' insulin resistance,to investigating mechanisms of TNAD on improving insulin sensitivity of adipose tissues.Methods Sixty male Sprague-Dawley(SD)rats of eight week were randomly divided into control group(n=10 rats)and IR group(n=50 rats)after 1 week adaptive feed.The rats in IR model group were fed with high fat diet and low-dose streptozotocin(STZ)to establish type 2 diabetes mellitus rat IR model.Then,according to the weight,the rats in IR model were randomly divided into high-,middle-,low-dose TNAD group,jinlida group and IR group,Rats in treated group were respectively given by corresponding-dose medicine gavage intervention treatment with high fat diets,Rats in model group were fed with a high fat diet and rats in control group with normal diet both of which were givened the same-dose saline solution gavage.After 4 weeks,rats were killed and taken 8ml abdominal aortic blood samples,perirenal adipose tissues.ELISA was conducted to measure the level of GSP,serum fins,leptin and adiponectin.The levels of fasting blood glucose,TC,TG,LDL-c and HDL-c were detected by biological and chemical method,calculated the HOMA-IR and IAI being calculated.HE staining was conducted to describe morphology changes of perirenal adipose tissues by the optical microscope,RT-PCR was adopeted to test expression of micro RNA-29 in adipose tissue.The date obtained using the Excel entry and used SPSS17.0 for date processing and analysis.Results 1 Glucose and lipid metabolism indexes: compared with the model group,the levels of FBG,GSP,Fins,HOMA-IR in all drug groups reduced in different degree(P<0.01),while the IAI level were increased obviously(P<0.01),the effects of TNAD middle-dose group in ameliorate the level of Fins,HOMA-IR,IAI was higher than others(P<0.01).2 Lipid metabolism indexes:compared with the model group,the level of TC,TG,LDL-c in all medicinal groups reduced inordinately(P<0.01),while level of HDLc increased obviously(P<0.01).3 Adipokines: compared with the model,the leptin level in all drug groups reduced(P<0.01),while the adiponectin level were increased(P<0.01),compared with the jinlida group,the effects of TNAD was stronger than jinlida in adjusting level of leptin and adiponectin(P<0.01).4 Morphology changes of adipose tissue:compared with control group,adipocyte's area of model group rats increased significantly,the boundary was not clear,some part showed tendency of fusion to a larger cell,more infiltration inflammatory cells and obvious congestion between adipocyte.After treatment,the area of adipocyte reduced significantly,and infiltration of inflammatory cells and the congestion was not obvious.5 Expression of micro RNA-29: compared with the control group,micro RNA-29 expression level in model increased markedly(P<0.05),compared with model group,the micro RNA-29 level in all medicinal groups reduced in different degree(P<0.01).compared with jinlida group,effects of TNAD low-and middle-dose groups were more obvious in reducting level of micro RNA-29(P<0.01).Conclusions 1 TNAD can obviously ameliorate the glucose and lipid metabolism disorder of IR rats.2 TNAD can relieve hypertrophy of adipocytes,infiltration of inflammatory cells and congestion reaction in the insulin sensitivity rats,ameliorating morphology changes of adioicyte in insulin resistance.3 Effect mechanisms of TNAD in ameliorating the IR is probably related to regulation on blood level of leptin,adiponectin and to inhibition on the overexpress of microRNA-29. |
PDF Full Text Request