Study On Anti-Lewis Lung Cancer Tumor By Jiedu Sanjie Capsule

Objective:Through the establishment of Lewis lung cancer tumor bearing mice model,the mice were given the corresponding drug treatment.The mice observed before and after treatment of life status,weight changes,tumor inhibition rate,thymus index,spleen index and immunohistochemical detection of the(VEGF)and microvessel density(MVD)in the tumor of mice were studied in order to explore the anti-tumor effect and mechanism of Jiedu Sanjie capsule and the clinical application of Jiedu Sanjie capsule provide a reliable scientific basis.Methods: Sixty healthy C57BL/6 mice with SPF grade were selected.The mice were randomly divided into blank group,model group,positive group(cyclophosphamide effective dose group),CTX group(cyclophosphamide critical inactive dose group)and Jiedu Sanjie high dose + CTX group,Jiedu Sanjie middle dose + CTX group,Jiedu Sanjie low dose + CTX group,Jiedu Sanjie high dose group(In order to ensure the feasibility and authenticity of the experiment,the model group,the positive group,the CTX group,the Jiedu Sanjie high dose + CTX group,Jiedu Sanjie middle dose +CTX group,Jiedu Sanjie low dose + CTX group,Jiedu Sanjie high dose group 9 mice per group,blank group 7 mice,the weight of each group of mice were no differences(P <0.05)between the body weight of mice).In addition to the blank group,the other groups of mice were right axillary injection of Lewis lung cancer cell suspension0.1ml,the second day after the model began to give the appropriate drug gavage.The quality of life of each group was observed at the same time,and the body weight of each group was weighed before the experiment and the end of the experiment.After14 days of intragastric administration,the mice were sacrificed and the rats were sacrificed and the thymus and spleen were quickly weighed.The tumor inhibition rate of each group was calculated,and the thymus index and spleen index of each group were calculated.The expression of vascular endothelial growth factor(VEGF)and microvessel density(MVD)in tumor tissues of tumor-bearing mice were detected by immunohistochemistry.Results:(1)The quality of life of the Jiedu Sanjie high dose + CTX group,the Jiedu Sanjie middle dose + CTX group,the Jiedu Sanjie low dose + CTX group and the Jiedu Sanjie high dose group were higher than those in the model group,positive group and CTX group.In the positive group,the quality of life of the mice was the worst,the hair removal,the coat color were sparse,dull,the body temperature was low,and the concentration of the czochrals was the earliest,indicating that the Jiedu Sanjie capsule could improve the quality of life of the tumor-bearing mice.(2)The body weight of the mice in the positive group decreased after the end of the experiment,and the body weight of the other groups was increased.Compared with the CTX group,the Jiedu Sanjie and astigmatism combined with the CTX group and the Jiedu Sanjie and trichoside group's weight gain were more obvious,indicating that Jiedu Sanjie capsules to a certain extent,by improving the weight of mice to improve the quality of life of mice.(3)The quality of the Jiedu Sanjie Capsule group was significantly lower than that of the model group(P<0.05).There was no significant difference between the TCM group and the CTX group(P> 0.05).and there was significant difference between the CTX group and Jiedu Sanjie high dose +CTX group and Jiedu Sanjie middle dose + CTX group(P <0.05).Indicating that the experiment Jiedu Sanjie capsules,although no direct anti-tumor effect,but can cooperate with chemotherapy drugs to increase the role of chemotherapy drugs.(4)Compared with the blank group,the thymus index decreased,while the spleen index increased,which may be due to inoculation of Lewis lung cancer cells,the mouse peripheral immune response increased,and central immune suppression was inhibited;positive group,CTX group thymus Index,spleen index were lower than the model group,indicating that cyclophosphamide can make the immune organs of mice atrophy;Chinese herbal medicine group of mice thymus,spleen index were higher than the positive group,CTX group,indicating that detoxification Sanjie capsules Inhibition of chemotherapy caused by immune organ atrophy.(5)The expression of VEGF,MVD and the expression of MVD in the low dose + CTX group,the Jiedu Sanjie and astigmatism dose group and the CTX group were significantly different from those in the CTX group and the model group.There was no significant difference between the high dose group and the CTX group and the model group,and the expression of VEGF and MVD in the positive group were significantly differentfrom those in the other groups,indicating that the Jiedu Sanjie capsule combined with chemotherapy VEGF,MVD expression play an anti-tumor effect.Conclusion:1 Jiedu Sanjie capsule can not inhibit tumor growth in Lewis lung cancer mice.2 Jiedu Sanjie capsuie has the effect of attenuating and enhancing the chamotherp--eutic drugs.3 Jiedu Sanjie capsules can improve the body of the thymus and spleen caused by chemotherapy drugs to reduce the body's immune response.4 Jiedu Sanjie capsule combined with chemotherapy can inhibit the growth of vasc--ular endothelial growth factor(VEGF)and microvessel density(MVD)in tumor tissue and play an anti-tumor effect.