The Study Of Antithrombotic Effects Of Kangxian Yi Xin Concentrated Pill

Objective:Through the establishment of inferior vena cava thrombus model in rats,observe Kangxian Yi xin concentrated pill^'effect on rat vein thrombosis;Through the monitoring of whole blood clotting time in mice,looking Kangxian Yi xin concentrated pill'effect on whole blood clotting time in mice;By detecting the rat blood,looking Kangxian Yi xin concentrated pill'effect on rat serum APTT,PT,TT,the influence of plasma TXB₂,6-keto-PGF1a.Respectively from the above four parts,comprehensive discuss Kangxian Yi xin concentrated pill'the antithrombotic effect of concentrated pill,perfect Kangxian Yi xin concentrated pill for new drug resistant.Methods:1 SD rats were randomly divided into Kangxian Yi xin concentrated pill'high doses?18.8 g/kg?,low dose group?4.7 g/kg?,sodium warfarin group?1.1 mg/kg?,and blank group?such as capacity of physiological saline?groups of animals in the same time lavage 1 times a day,7 d continuous lavage.Lavage according to deep vein thrombosis after animal model building,4 h after open abdominal cavity,abdominal aorta take blood tests,APTT,TT,PT content in inferior vena cava thrombus,said its wet weight,24 h in 60?oven drying,weigh and dry weight.2 Kunming mice were randomly divided into Kangxian Yi xin concentrated pill'high?27.1 g/kg?,low dose group?6.8 g/kg?,aspirin,the control group?20 mg/kg?,and blank control group?such as capacity of physiological saline?groups of animals in the daily at around 8 am lavage,continuous lavage in 3 d,the capillary method is adopted to observe each group clotting time.3 SD rats were randomly divided into Kangxian Yi xin concentrated pill'high doses?18.8 g/kg?,low dose group?4.7 g/kg?,aspirin group?20 mg/kg?,blank control group?amount of saline?each animal feeding at around 8 am 1 times a day,7 d continuous lavage.Abdominal aorta in blood sodium heparin anticoagulation,specimen collection within 30 min 3000 r.centrifugal 15 min,take the supernatant,using the ELISA method for the determination of plasma 6-keto-PGF1a,TXB₂level.Results:1 Compared with the blank control group,high and low dose group and warfarin sodium could significantly reduce rats formed by inferior vena cava ligation of the wet and dry weight of thrombus?P<0.05?,prolong APTT,PT,TT?P<0.05?among them,the high dose group and warfarin sodium effect more obvious?P<0.01?;High dose group compared with warfarin sodium group,role,there was no significant difference?P>0.05?.2 Compared with the blank control group,high and low dose group and control group were significantly prolong clotting time,aspirin was statistically significant?P<0.05?.High and low dose group compared with the blank group,there are obvious differences,significantly extended,clotting time?P<0.05?.Between high and low dose group and aspirin in the control group,there are differences?P<0.05?,high and low dose group compared with aspirin,clotting time shorter than aspirin in the control group.There are differences between the high dose group and low dose group?P<0.05?,high dose group of blood coagulation time longer than the low dose group.3 Compared with the blank control group,high dose group,aspirin group significant difference?P<0.05?,has obvious statistical significance;The difference is not obvious in the lower dosage treatment groups?P>0.05?.High dose group has obviously improved 6-keto-PGF1a value,reduce TXB₂ value,the effect of low dose though statistically insignificant,but improved 6-keto-PGF1a value,reduce TXB₂value trendConclusion:1 Kangxian Yi xin concentrated pill can inhibit the formation of venous thrombosis,which indicates that it has a certain resistance to the effect of venous thrombosis.2 Kangxian Yi xin concentrated pill has extended of APTT and PT,TT,shows that Kangxian Yi xin concentrated pill has antithrombotic effect,this may be through the influence on blood coagulation process and play a role of antithrombotic.3 Kangxian Yi xin concentrated pill can prolong the clotting time,it shows that Kangxian Yi xin concentrated pill has antithrombotic effect,it may be based on the influence of the endogenous clotting in the process of coagulation process and play a role of antithrombotic.4 Kangxian Yi xin concentrated pill can reduce TXB₂,6-keto-PGF1a increases,it shows that Kangxian Yi xin concentrated pill have certain antithrombotic effect,this effect may be associated with platelet aggregation function and endothelial function...