Effects Of Different Feeding Modes On Rumen Fermentation And Nutrient Metabolism In Sheep

The study was designed with 3 x 3 Latin square.Six sheep with permanent rumen fistulae were selected as test animals and fed in different feeding modes respectively.The experiment was divided into three phases.The sheep in the control group was fed with traditional feeding mode that fine after the roughage feeding mode(roughage and fine feed are separated by 30 min),The group of total mixed ration(TMR)was feed with total mixed ration.The group of TMR particles group was feed with total mixed ration particles feed.Each trial for 17 days,including pre-trial period of 10 d,the trial period of 7 d,with a total of 51 d.The nylon bag method was used to determine three diet at different time points in sheep rumen degradation rate and To determine the effects of different feeding patterns on rumen fermentation index,apparent digestibility and blood biochemical indexes of sheep.The experimental results showed that:(1)Different feeding patterns had no significant effect on the average values of rumen pH,ammonia nitrogen,acetic acid,propionic acid,ethylene/propylene ratio,butyric acid,and bacterial protein(P>0.05).The pH of the TMR pellet group was significantly higher than that of the other two groups(P<0.05)and the concentration of acetic acid in the TMR pellet group was significantly lower than that in the traditional group and the TMR group(P<0.05)at 6 h and 9 h.At 12 h,the concentration of propionic acid in the TMR pellet group was significantly lower than that in the traditional group and the TMR group(P<0.05).At 6 h and 9 h,the concentration of butyrate in the TMR pellet group and the traditional group was significantly lower than that in the TMR group(P<0.05).TMR particle group Acetic acid than propionic acid were significantly lower than those of traditional and TMR groups(P<0.05)at 6 h.At 12 h,the bacterial protein content in the TMR granule group and the TMR group was significantly lower than that in the traditional group(P<0.05).(2)At 24 h and 48 h,the degradation rates of dry matter and NDF of the TMR and TMR groups were significantly higher than that in the traditional group(P<0.05).At 12 h,the degradation rate of ADF in the TMR and TMR groups was significantly higher than that in the traditional group(P<0.05).There were no significant differences in degradable fraction,fast degraded fraction,slow degraded fraction and effective degradation rate of DM,NDF,ADF in the rumen between the treatment groups(P>0.05).(3)There was no significant effect on the apparent digestibility of dry matter,organic matter and crude fat in sheep(P>0.05).The apparent digestibility of crude protein and acid detergent fibers in the TMR particles group was significantly higher than that in the other two groups(P<0.05).The apparent digestibility of neutral detergent fiber in TMR particles group and traditional group was significantly higher than that in TMR group(P<0.05).(4)There was no significant difference on the content of aspartate aminotransferase,alanine aminotransferase,total protein,albumin,globulin,triacylglycerol,and urea nitrogen in sheep serum(P>0.05).The glucose concentration in the TMR pellet group was significantly higher than that in traditional group(P<0.05),the total cholesterol content in the traditional group and the TMR group was significantly higher than that in the TMR pellet group(P< 0.05).On the whole,compared with the traditional feeding mode and TMR feeding mode,TMR particles feeding mode can guarantee the rumen fermentation of sheep and make the rumen more stable.Improve the digestibility of sheep crude protein and fiber,increase the glucose content an dreduce the cholesterol content in sheep blood.Therefore,it can effectively promote the digestio n and absorption of nutrients by sheep,thus improving the feeding effect of sheep.