The Effect Of Different Forage Quality On Rumen Fermentation And Microflora Of Sheep

Nine healthy Inner Mongolian semi-fine wool sheep,fitted with permanent ruminal and proximal duodenum fistula. The paper mainly studied the effect of different forage quality grading index on rumen fermentation and microflora of sheep .The experiment was composed of four parts.Partâ… :Diets were divided into three groups by using Model of Forage Grading Index Parameters, namely high forage grading index (HGI) group,middle forage grading index(MGI) group and low forage grading index(LGI) group;Partâ…¡:the degradability of forage DM and NDF in the rumen of sheep were determined by in situ technique;Partâ…¢:the rumen environment change and the Flow rule of nutrition substance of rumen and duodenum were determined at different three diets;Partâ…£:The relative amount of three ruminal predominant cellulolytic bacteria (Ruminococcus flavefaciens, Ruminococcus albus and Fibrobacter succinogenes) were determined by nylon membrane fleck Blotting using oligonecleotide probes of 16SrRNA.The results indicated: Grading index value of LGI group,MGI group and HGI group were 0.17,0.36 and 1.22MJ respectively;The change trend of degradability of forage DM was LGI0.05). For LGI group,MGI group and HGI group ,The relative percentage of F.suc and R.alb in total bacteria amounts were 10.01%,7.90%,11.92%and3.19%,5.24%,6.30%respectively. But MGI group was higher than HGI and LGI group on the relative percentage of R.fla .in total bacteria amounts. High forage grading index was higher relative percentage of Fibrobacter succinogenes,Ruminococcus albus and total cellulolytic bacteria(P>0.05).From above: rumen microorganisms varied with diets of different forage grading index.