Effect Of Xylooligosaccharide On Intestinal Mucosal Barrier Function In Stressed Rats

Objective:To study the effect of the xylo—oligosaccharide(XOS) added in the diet on the injured intestinal mucous membrane barrier caused by electricity restraint stress.Methods : Forty wistar rats were randomly divided into groups:two electric resistant stress(ERS)groups fed with either a normal diet(ERS group) or 2g/kg XOS per day(ERS+XOS group) and two sham ERS groups fed with a normal diet(control group) or2g/kg XOS per day(XOS group).All the four groups were fed for a total of 26 days, and the ERS and ERS+XOS groups were subjected to electric restraint stress starting from the12 th day,for 2 weeks. Then the blood samples of all the four groups were collected via abdominal aorta puncture for determination of serum D-lactate and diamineoxidas(DAO)activity; finally all the rats were sacrificed and the intestinal tissues were collected for examination of pathological tissue, membrane morphology and SIgA contents.Results:Compared with the control, the serum D-lactate and DAO levels of ERS group and the ERS+XOS group were significantly increased and positively correlated(p<0.05);and that in the ERS group was significantly higher than that in the ERS+XOS group(p<0.05); the levels of serum D-lactate and DAO were not significantly different between the control and the XOS groups(p>0.05). The parameter values of intestinal membrane morphology and SIgA contents of the ERS group and ERS+XOS group were obviously declined by comparison with the control group(p<0.05); and that in the ERS group was significantly decreased than that in the ERS+XOS group(p<0.05); but that in the ERS+XOS group were significantly increased than that in the control group(p<0.05).Conclusion:Feeding with XOS can decrease the serum concentration of D-lactate and DAO in rats and improve intestinal mucosa morpology and function, suggesting that XOS may have to maintain intestinal barrier function.