The Functional Change Of Intestinal Mucosa Barrier In Patients With Parkinson’s Disease

Background and ObjectiveNeurological diseases are the major source of disability worldwide,and Parkinson disease(PD)is the fastest growing neurological disease in the world.The pathogenesis of Parkinson’s disease is not yet clear.Studies have suggested that oxidative stress and mitochondrial dysfunction,protein aggregation and misfolding,neuroinflammation,excitotoxicity,apoptosis and loss of cell death pathways and nutrient factors may be involved.Typical pathological changes of PD include degeneration and death of dopaminergic neurons in the dense part of the substantia nigra of the midbrain,as well as lewy bodies and lewy body protrusions composed of misfolded synaptic ribosomal protein aggregates,which gather in neurons in the form of lewy bodies and lewy body protrusions.Studies have shown that boss-synaptic nuclear proteins are not only present in the central nervous system,but also expressed in the intestinal nervous system.Therefore,biomarkers in the intestinal nervous system play an important role in the diagnosis of diseases.The levels of serum Diamine oxidase(DAO),D-lactate(D-LA)and Lipopolysaccharide(LPS)are sensitive indicators that indirectly reflect the function of early intestinal mucosal barrier and intestinal mucosal permeability,so the changes which DAO,D-LA and LPS levels in peripheral blood may provide a basis for the diagnosis of PD.Hoehn-yahr(H-Y)grading is mainly based on the clinical symptoms and signs of PD patients,and is an important indicator to evaluate the severity of the disease.In this study,the serum indexes DAO,D-LA and LPS of intestinal mucosal barrier in PD patients were investigated,as well as the correlation with the severity of PD.This study is expected to provide a basis for the early diagnosis of PD.Material and methodsFrom January 2019 to December 2019,48 patients with Parkinson’s disease who were admitted to the second affiliated hospital of zhengzhou university were selected as the experimental group(PD group).At the same time,40 healthy physical examinees matching gender and age were selected as the control group(NPD group).Blood samples of PD patients and healthy people were collected and the levels of DAO,D-LA and LPS were detected.SPSS20.0 software was used for statistical analysis of the research data.Results1.The levels of DAO,D-LA and LPS in PD group were(14.01 ±2.87)U/L,(25.85±4.56)mg/L and(21.92±3.41)U/L,respectively,which were all higher than those in NPD group(10.62±1.59)U/L,(22.53±4.32)mg/L and(17.85±2.94)U/L,with statistically significant differences(P<0.05).2.The correlation between H-Y grading and serum DAO,D-LA and LPS levels in PD patients was analyzed.The results showed that H-Y grading in PD patients was positively correlated with DAO(r=0.877,P<0.05),D-LA(r=0.760,P<0.05)and LPS(r=0.414,P<0.05).3.The ROC curve showed that the decline area of the curve of PD predicted by DAO was 0.846,the sensitivity was 79.2%,and the specificity was 80.0%.D-LA predicted that the drop area of PD curve was 0.760,the sensitivity was 77.1%,and the specificity was 72.0%.The decline area of PD curve predicted by LPS was 0.822,the sensitivity was 87.5%,and the specificity was 67.5%.ConclusionsThe levels of Diamine oxidase,D-lactic and Lipopolysaccharide are positively correlated with the severity of Parkinson’s disease in patients with intestinal mucosal barrier dysfunction,which has certain clinical significance for the disease evaluation and diagnosis of Parkinson’s disease.